Chen X C, Xia L, Hu S, Huang G
Department of Pharmacology, Hainan Medical College, Haikou, China.
Zhongguo Yao Li Xue Bao. 1996 Nov;17(6):523-6.
To study the effects of Acanthopanax gracilistylus var pubescens Li saponins (AGVPS) on human platelet aggregation and platelet factor 4 (PF4) liberation in vitro.
Human platelet aggregations induced by ADP, adrenaline, and collagen were measured turbidimetrically. The aggregation curve was recorded on a platelet aggregometer and the maximal aggregation rate (ARmax), effective deaggregation rate in 5 min (DR5 min) and lag time (LT) were autocalculated by the built-in microcomputer; PF4 liberation from human platelets stimulated by ADP and collagen was determined by recording the heparin thrombin clotting time (HTCT). Thrombosis was tested by weighing the wet and dry thrombi formed in a siliconized revolving ring.
AGVPS inhibited in vitro the ARmax with IC50 of 1.33 (95% confidence limits: 1.09-1.63, ADP-induced), 1.66 (1.54-1.79, adrenaline-induced), and 4.2 g.L-1 (0.6-29, collagen-induced). The DR5 min (on ADP-induced aggregation) and LT (collagen-induced) were also increased as well. Meanwhile, AGVPS 0.63-2.50 g.L-1 prolonged HTCT on ADP- and collagen-stimulated PF4 liberation. At 0.34-1.39 g.L-1, AGVPS reduced the wet and dry weight of thrombi formed in vitro.
AGVPS inhibits human platelet aggregation, liberation, and thrombosis in vitro, suggesting its possible antithrombotic action in man.
研究细柱五加变种柔毛五加皂苷（AGVPS）对人血小板聚集及血小板因子4（PF4）体外释放的影响。
采用比浊法测定AGVPS对二磷酸腺苷（ADP）、肾上腺素和胶原诱导的人血小板聚集的影响。用血小板聚集仪记录聚集曲线，内置微机自动计算最大聚集率（ARmax）、5分钟有效解聚率（DR5min）和延迟时间（LT）；通过记录肝素-凝血酶凝血时间（HTCT），测定AGVPS对ADP和胶原刺激的人血小板PF4释放的影响。通过称量在硅化旋转环中形成的湿血栓和干血栓重量来检测血栓形成。
AGVPS体外抑制ARmax，ADP诱导时IC50为1.33（95%置信限：1.09 - 1.63），肾上腺素诱导时为1.66（1.54 - 1.79），胶原诱导时为4.2 g.L-1（0.6 - 29）。同时，AGVPS还增加了（ADP诱导聚集时的）DR5min和（胶原诱导时的）LT。此外，0.63 - 2.50 g.L-1的AGVPS延长了ADP和胶原刺激的PF4释放时的HTCT。在0.34 - 1.39 g.L-1时，AGVPS降低了体外形成血栓的湿重和干重。
AGVPS体外抑制人血小板聚集、释放及血栓形成，提示其在人体内可能具有抗血栓作用。
