Chen X M, Pan J Q, Zhang Z N
Division of Hematology, Peking Union Medical College Hospital, Beijing, China.
Zhongguo Yao Li Xue Bao. 1995 May;16(3):226-30.
To study the effects of propylene glycol mannate sulfate (PGMS) on platelet adhesion, aggregation and thrombosis in abdominal aorta in rabbits.
Platelet adhesion assay was performed with a platelet-adhesion-meter. The platelet concentration was determined with an electronic particle counter, and the total radio-activity was determined with Clinigamma-1272.
PGMS inhibited washed platelet aggregation induced by thrombin in vitro. The value of IC50 was 0.9 mg.L(-1). (95% confidence limit = 4.1-13.7 mg.L(-1). At 10, 30, and 60 min after i.v. PGMS 75 mg.kg(-1), the inhibition rates of platelet adhesion were 90.4%, 41.8%, and 26.3% and the inhibition rates of platelet aggregation induced by thrombin were 99%, 122%, and 110%, respectively. It did not exhibit any noticeable effect on platelet aggregation induced by ADP and collagen at this dosage. After 1.5 h of i.v. PGMS 75 mg.kg-1 and total autologous 111In-platelets 3.3 x 10(9) the radioactivity and dry weight of the injured and uninjured segments were determined. The PGMS group showed a significantly lower radioactivity (1.1 +/- 0.6 MBq), 111In-platelets (8.3 +/- s 3.3 x 10(8)) as well as the radioactivity deposited.g(-1) out of the total radioactivity infused % (0.24 +/- 0.21) compared with the control group (3.7 +/- 0.5 MBq, 24.6 +/- 3.5 x 10(8), and 0.86 +/- 0.25, respectively).
PGMS prevented the platelet adhesion and aggregation at the injured arterial wall.
研究硫酸丙酯甘露聚糖（PGMS）对兔腹主动脉血小板黏附、聚集及血栓形成的影响。
采用血小板黏附仪进行血小板黏附试验。用电子粒子计数器测定血小板浓度，用Clinigamma - 1272测定总放射性。
PGMS在体外可抑制凝血酶诱导的洗涤血小板聚集。半数抑制浓度（IC50）值为0.9mg·L⁻¹（95%可信限 = 4.1 - 13.7mg·L⁻¹）。静脉注射PGMS 75mg·kg⁻¹后10、30和60分钟，血小板黏附抑制率分别为90.4%、41.8%和26.3%，凝血酶诱导的血小板聚集抑制率分别为99%、122%和110%。在此剂量下，对ADP和胶原诱导的血小板聚集未表现出明显作用。静脉注射PGMS 75mg·kg⁻¹ 1.5小时后，注入3.3×10⁹个自体¹¹¹In标记血小板，测定损伤段和未损伤段的放射性及干重。与对照组相比，PGMS组的放射性（1.1±0.6MBq）、¹¹¹In标记血小板数（8.3±3.3×10⁸）以及注入总放射性中沉积的放射性占比（0.24±0.21）显著降低（对照组分别为3.7±0.5MBq、24.6±3.5×10⁸和0.86±0.25）。
PGMS可防止血小板在损伤动脉壁的黏附和聚集。
