硫酸甘露聚糖丙二醇酯对兔腹主动脉血栓形成的影响。

Effects of propylene glycol mannate sulfate on thrombosis in abdominal aorta in rabbits.

作者信息

Chen X M, Pan J Q, Zhang Z N

机构信息

Division of Hematology, Peking Union Medical College Hospital, Beijing, China.

出版信息

Zhongguo Yao Li Xue Bao. 1995 May;16(3):226-30.

PMID:7660816

Abstract

AIM

To study the effects of propylene glycol mannate sulfate (PGMS) on platelet adhesion, aggregation and thrombosis in abdominal aorta in rabbits.

METHODS

Platelet adhesion assay was performed with a platelet-adhesion-meter. The platelet concentration was determined with an electronic particle counter, and the total radio-activity was determined with Clinigamma-1272.

RESULTS

PGMS inhibited washed platelet aggregation induced by thrombin in vitro. The value of IC50 was 0.9 mg.L(-1). (95% confidence limit = 4.1-13.7 mg.L(-1). At 10, 30, and 60 min after i.v. PGMS 75 mg.kg(-1), the inhibition rates of platelet adhesion were 90.4%, 41.8%, and 26.3% and the inhibition rates of platelet aggregation induced by thrombin were 99%, 122%, and 110%, respectively. It did not exhibit any noticeable effect on platelet aggregation induced by ADP and collagen at this dosage. After 1.5 h of i.v. PGMS 75 mg.kg-1 and total autologous 111In-platelets 3.3 x 10(9) the radioactivity and dry weight of the injured and uninjured segments were determined. The PGMS group showed a significantly lower radioactivity (1.1 +/- 0.6 MBq), 111In-platelets (8.3 +/- s 3.3 x 10(8)) as well as the radioactivity deposited.g(-1) out of the total radioactivity infused % (0.24 +/- 0.21) compared with the control group (3.7 +/- 0.5 MBq, 24.6 +/- 3.5 x 10(8), and 0.86 +/- 0.25, respectively).

CONCLUSION

PGMS prevented the platelet adhesion and aggregation at the injured arterial wall.

摘要

目的

研究硫酸丙酯甘露聚糖（PGMS）对兔腹主动脉血小板黏附、聚集及血栓形成的影响。

方法

采用血小板黏附仪进行血小板黏附试验。用电子粒子计数器测定血小板浓度，用Clinigamma - 1272测定总放射性。

结果

PGMS在体外可抑制凝血酶诱导的洗涤血小板聚集。半数抑制浓度（IC50）值为0.9mg·L⁻¹（95%可信限 = 4.1 - 13.7mg·L⁻¹）。静脉注射PGMS 75mg·kg⁻¹后10、30和60分钟，血小板黏附抑制率分别为90.4%、41.8%和26.3%，凝血酶诱导的血小板聚集抑制率分别为99%、122%和110%。在此剂量下，对ADP和胶原诱导的血小板聚集未表现出明显作用。静脉注射PGMS 75mg·kg⁻¹ 1.5小时后，注入3.3×10⁹个自体¹¹¹In标记血小板，测定损伤段和未损伤段的放射性及干重。与对照组相比，PGMS组的放射性（1.1±0.6MBq）、¹¹¹In标记血小板数（8.3±3.3×10⁸）以及注入总放射性中沉积的放射性占比（0.24±0.21）显著降低（对照组分别为3.7±0.5MBq、24.6±3.5×10⁸和0.86±0.25）。