在酿酒酵母中，是APT1而非APT2编码一种功能性腺嘌呤磷酸核糖转移酶。
APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae.
作者信息
Alfonzo J D, Crother T R, Guetsova M L, Daignan-Fornier B, Taylor M W
机构信息
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
出版信息
J Bacteriol. 1999 Jan;181(1):347-52. doi: 10.1128/JB.181.1.347-352.1999.
Abstract
The yeast Saccharomyces cerevisiae has two separate genes (APT1 and APT2) that encode two potentially different forms of adenine phosphoribosyltransferase (APRT). However, genetic analysis indicated that only APT1 could code for a complementing activity. Cloning and expression of both the APT1 and APT2 genes in Escherichia coli showed that although discrete proteins (APRT1 and APRT2) were made by these genes, only APRT1 had detectable APRT activity. Northern and Western blot analyses demonstrated that only APT1 was transcribed and translated under normal physiological conditions in yeast. Phylogenetic analysis revealed that APRT1 and APRT2 are evolutionary closely related and that they arise from a gene duplication event. We conclude that APT1 is the functional gene in S. cerevisiae and that APT2 is a pseudogene.
摘要
酿酒酵母有两个独立的基因（APT1和APT2），它们编码两种潜在不同形式的腺嘌呤磷酸核糖转移酶（APRT）。然而，遗传分析表明只有APT1能够编码一种互补活性。将APT1和APT2基因在大肠杆菌中进行克隆和表达，结果显示虽然这些基因产生了不同的蛋白质（APRT1和APRT2），但只有APRT1具有可检测到的APRT活性。Northern印迹和Western印迹分析表明，在酵母正常生理条件下，只有APT1被转录和翻译。系统发育分析表明，APRT1和APRT2在进化上密切相关，它们源自一次基因复制事件。我们得出结论，APT1是酿酒酵母中的功能基因，而APT2是一个假基因。
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