Abernathy T V, Lee K B, Parker R J, Reed E
Medicine Branch, Division of Clinical Sciences, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA.
Oncol Rep. 1999 Jan-Feb;6(1):155-9.
Cadmium (Cd), a toxic heavy metal and probable carcinogen in humans, appears to have potential anti-cancer activity in pre-clinical systems. This observation led us to develop a method for measuring cellular Cd and DNA-bound Cd following micromolar exposures to cadmium dichloride. Cultured human ovarian cancer cell lines were used. Following low level exposures to cadmium dichloride (CdCl2), atomic absorption spectrometry with Zeeman background correction was used to measure total cell associated Cd in wet-ashed cells, and the lower limits of detection was at 100 pg of Cd per 106 cells. In cellular DNA isolated by cesium chloride density gradient centrifugation, levels of 1.5 Cd lesions (Cd molecules) per 106 nucleotides were reproducibly detected. Standard curves with samples yielded 76.4 6.7% recovery when using picogram quantities of Cd. Manipulation of the total amount of biological material used, can further improve detection limits. Thus, this method is suitable for the detection of Cd in biological matrixes after low levels of Cd exposure, and shows good performance in terms of the level of sensitivity and reproducibility.
镉（Cd）是一种有毒重金属，对人类可能具有致癌性，但在临床前系统中似乎具有潜在的抗癌活性。这一观察结果促使我们开发一种方法，用于在微摩尔浓度的二氯化镉暴露后测量细胞中的镉和与DNA结合的镉。使用培养的人卵巢癌细胞系。在低水平暴露于二氯化镉（CdCl2）后，采用带有塞曼背景校正的原子吸收光谱法测量经湿灰化处理的细胞中与细胞相关的总镉，检测下限为每106个细胞100皮克镉。在通过氯化铯密度梯度离心分离的细胞DNA中，每106个核苷酸可重复检测到1.5个镉损伤（镉分子）的水平。当使用皮克量的镉时，样品的标准曲线回收率为76.4±6.7%。控制所用生物材料的总量可进一步提高检测限。因此，该方法适用于低水平镉暴露后生物基质中镉的检测，在灵敏度和重现性方面表现良好。
