Shrestha M L, Miyake H, Kikutsuji T, Tashiro S
First Department of Surgery, University of Tokushima School of Medicine, Japan.
J Med Invest. 1998 Aug;45(1-4):95-102.
Ki-67 and p53 protein expression was evaluated immunohistochemically in 32 patients with intrahepatic, extrahepatic bile duct and gallbladder carcinomas, who underwent surgery at First Department of Surgery, The University of Tokushima School of Medicine. p53 expression was found more in the well differentiated group than poorly differentiated group (p = 0.007). MIB1 labelling index (MIB1 LI) was higher in EHC than in GBC (p = 0.0061). MIB1 LI (T), (MIB1 LI in tumor) was higher in cases with lymph node metastasis than in those without lymph node metastasis (p = 0.0189). Moreover, MIB1 LI (L) (MIB1 LI in metastasized lymph node) was higher in poorly differentiated than in well differentiated carcinoma (p = 0.0404). Prognostically, patients with high MIB1 LI (T) (> 56.93) had a worse prognosis after surgery than those with low MIB1 LI (T) (p < 0.05). There was no association between p53 positive tumors and MIB1 expression. These results suggest that cancer cell proliferative activity was markedly increased in cases with EHC compared to those with GBC and the poorly differentiated and lymph node metastasis group. MIB1 LI in tumor was found to be a good prognostic indicator whereas there was no association of p53 positive tumor with MIB1 expression and prognosis of the patients.
对32例肝内、肝外胆管及胆囊癌患者的Ki-67和p53蛋白表达进行了免疫组化评估，这些患者均在德岛大学医学院第一外科接受了手术。发现p53在高分化组中的表达多于低分化组（p = 0.007）。肝外胆管癌（EHC）的MIB1标记指数（MIB1 LI）高于胆囊癌（GBC）（p = 0.0061）。有淋巴结转移的病例中肿瘤的MIB1 LI（T）高于无淋巴结转移的病例（p = 0.0189）。此外，低分化癌中转移淋巴结的MIB1 LI（L）高于高分化癌（p = 0.0404）。预后方面，MIB1 LI（T）高（> 56.93）的患者术后预后比MIB1 LI（T）低的患者差（p < 0.05）。p53阳性肿瘤与MIB1表达之间无关联。这些结果表明，与胆囊癌以及低分化和有淋巴结转移组相比，肝外胆管癌患者的癌细胞增殖活性明显增加。肿瘤中的MIB1 LI是一个良好的预后指标，而p53阳性肿瘤与MIB1表达及患者预后无关联。
