Pfyffer G E, Auckenthaler R, van Embden J D, van Soolingen D
Swiss National Center for Mycobacteria, Department of Medical Microbiology, University of Zurich, Switzerland.
Emerg Infect Dis. 1998 Oct-Dec;4(4):631-4. doi: 10.3201/eid0404.980414.
An exceptionally smooth and glossy morphotype of Mycobacterium tuberculosis complex was isolated from a 56-year-old Swiss patient with mesenteric tuberculosis. Direct 16S rRNA sequence analysis of the hypervariable signature gene regions revealed a 100% homology to the specific M. tuberculosis complex sequence. Spoligotyping and restriction fragment length polymorphism analyses using the insertion sequences IS6110 and IS1081 and the polymorphic GC-rich sequence as additional genetic markers identified the isolate as the novel taxon M. canettii. Like a Somali child with a similar case, this patient probably contracted the infection in Africa, which raises questions about the geographic distribution of M. canettii.
从一名患有肠系膜结核的56岁瑞士患者体内分离出一种形态异常光滑且有光泽的结核分枝杆菌复合群。对高变特征基因区域进行直接16S rRNA序列分析，结果显示其与结核分枝杆菌复合群的特定序列具有100%的同源性。使用插入序列IS6110和IS1081以及富含GC的多态性序列作为额外的遗传标记进行寡核苷酸分型和限制性片段长度多态性分析，确定该分离株为新分类单元卡内蒂分枝杆菌。与一名患有类似病例的索马里儿童一样，该患者可能是在非洲感染的，这引发了关于卡内蒂分枝杆菌地理分布的问题。
