Jones B H, Standridge M K, Claycombe K J, Smith P J, Moustaïd-Moussa N
Department of Nutrition, University of Tennesse, Knoxville, TN 37996-1900, USA.
Biochem J. 1998 Oct 15;335 ( Pt 2)(Pt 2):405-8. doi: 10.1042/bj3350405.
Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is a key enzyme in the synthesis polyunsaturated fatty acids. Liver and ose tissue are the predominant sites of SCD expression. Regulation of tic SCD by various nutritional and hormonal ors, such as insulin, dietary carbohydrates and polyunsaturated fatty s, has been well documented. Little is known, ver, about adipocyte SCD regulation despite high levels of SCD activity adipose tissue. The present study was gned to investigate SCD regulation in adipocytes by examining the cts of glucose and insulin on SCD expression. We rt here that glucose availability directly increased SCD gene scription in 3T3-L1 adipocytes. This response was pendent of insulin, and insulin alone in the absence of glucose had no ct on SCD mRNA levels. SCD thus represents a l model in which to investigate the mechanisms of direct regulation of expression by glucose in adipose cells.
硬脂酰辅酶A去饱和酶（SCD；EC 1.14.99.5）是合成多不饱和脂肪酸的关键酶。肝脏和脂肪组织是SCD表达的主要部位。各种营养和激素因素，如胰岛素、膳食碳水化合物和多不饱和脂肪酸对SCD的调节作用已有充分记载。然而，尽管脂肪组织中SCD活性很高，但关于脂肪细胞SCD的调节却知之甚少。本研究旨在通过检测葡萄糖和胰岛素对SCD表达的影响来研究脂肪细胞中SCD的调节。我们在此报告，葡萄糖的可利用性直接增加了3T3-L1脂肪细胞中SCD基因的转录。这种反应不依赖于胰岛素，且在无葡萄糖的情况下单独使用胰岛素对SCD mRNA水平没有影响。因此，SCD代表了一个研究葡萄糖在脂肪细胞中直接调节基因表达机制的理想模型。
