Gu T, Niu X, Cheng D
Department of Plastic Surgery, Bethune International Peace Hospital, Shijiazhuang, P.R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 1997 Sep;11(5):261-3.
To investigate the best way of using fibroblast growth factor (FGF) in wound healing, the following experiments were performed. Twelve Wistar rats were chosen. Four 1.5 cm x 1.5 cm middle-thick skin wounds were made in the back of each rat, 2 in each side, and labelled as number 1 to 4. Number 1 wound of each rat was used as control, only PBS was applied to the wound, 50 microliters per time, twice a day from the first day to 11th day. Number 2 wound was sustained medication group, 50 microliters 4 micrograms/ml FGF was applied twice a day from the first day to 11th day; Number 3 wound was early medication group, 50 microliters 8 micrograms/ml FGF was applied twice a day from the first day to 5th day; Number 4 wound was late medication group, 50 microliters 8 micrograms/ml FGF was added twice a day from the 5th day to 11th day. By day 4, 8, 12 and 16, the area of wounds were measured, and the healing time of each wound was recorded. The elastic fiber, collagen fiber and DNA content were measured by immunohistological method. The result showed that the elastic fiber, collagen fiber and DNA content in the groups of FGF used were more than those in the control group. The healing time of the control group was 14.4 days while that of the early meduation group was 13.4 days, late medation group was 13.5 days and sustained medication group was 12.2 days. It was suggested that FGF could accelerate the wound healing, and sustained use of FGF was the best way of giving the drug.
为研究成纤维细胞生长因子（FGF）在伤口愈合中的最佳使用方式，进行了以下实验。选取12只Wistar大鼠。在每只大鼠背部制作4个1.5 cm×1.5 cm的中厚皮肤伤口，每侧2个，标记为1至4号。每只大鼠的1号伤口作为对照，仅在伤口处涂抹PBS，每次50微升，从第一天至第11天每天涂抹两次。2号伤口为持续给药组，从第一天至第11天每天两次涂抹50微升4微克/毫升的FGF；3号伤口为早期给药组，从第一天至第5天每天两次涂抹50微升8微克/毫升的FGF；4号伤口为晚期给药组，从第5天至第11天每天两次添加50微升8微克/毫升的FGF。在第4、8、12和16天测量伤口面积，并记录每个伤口的愈合时间。通过免疫组织学方法测量弹性纤维、胶原纤维和DNA含量。结果显示，使用FGF的组中弹性纤维、胶原纤维和DNA含量均高于对照组。对照组的愈合时间为14.4天，早期给药组为13.4天，晚期给药组为13.5天，持续给药组为12.2天。提示FGF可加速伤口愈合，持续使用FGF是给药的最佳方式。
