通过气相色谱法对人和各种动物全血中的乙醛进行定量分析。

Quantitative analysis of acetaldehyde in whole blood from human and various animals by gas chromatography.

作者信息

Miyake T, Shibamoto T

机构信息

Department of Environmental Toxicology, University of California, Davis 95616, USA.

出版信息

J Chromatogr B Biomed Sci Appl. 1998 Nov 20;719(1-2):213-6. doi: 10.1016/s0378-4347(98)00390-9.

DOI:10.1016/s0378-4347(98)00390-9

PMID:9869382

Abstract

Acetaldehyde present in the blood of bull, chicken, hamster, horse, human, monkey, pig, rabbit, rat and sheep, was quantitatively analyzed by a newly developed gas chromatographic method. Acetaldehyde in a blood sample was reacted with cysteamine to give 2-methylthiazolidine, which was extracted with dichloromethane and subsequently analyzed by gas chromatography with a fused-silica capillary column and a nitrogen-phosphorus detector. The quantities of acetaldehyde found in blood ranged from 2.04 micromol/ml (hamster) to 14.8 micromol/ml (pig). The quantity of acetaldehyde recovered from human blood was 6.17 micromol/ml.

摘要

采用新开发的气相色谱法对公牛、鸡、仓鼠、马、人、猴、猪、兔、大鼠和绵羊血液中的乙醛进行了定量分析。血液样本中的乙醛与半胱胺反应生成2-甲基噻唑烷，用二氯甲烷萃取后，再用带有熔融石英毛细管柱和氮磷检测器的气相色谱仪进行分析。血液中乙醛的含量范围为2.04微摩尔/毫升（仓鼠）至14.8微摩尔/毫升（猪）。从人血液中回收的乙醛量为6.17微摩尔/毫升。