用于检测日本人群中GB病毒C/庚型肝炎病毒RNA的5'非翻译区和NS3R引物的比较研究

Comparative study of 5' UTR and NS3R primers for the detection of GB virus C/hepatitis G virus RNA in Japanese.

作者信息

Tanaka H, Miyano M, Ueda H, Doi R, Mimura K, Nishide I, Yukawa S

机构信息

Third Department of Internal Medicine, Wakayama Medical College, Wakayama City, Japan.

出版信息

Liver. 1998 Dec;18(6):378-82. doi: 10.1111/j.1600-0676.1998.tb00821.x.

DOI:10.1111/j.1600-0676.1998.tb00821.x

PMID:9869391

Abstract

AIMS/BACKGROUND: Many epidemiological studies of new hepatitis viruses, including GB virus C (GBV-C) and hepatitis G virus (HGV), have used polymerase chain reaction (PCR) primers designed for the third nonstructural region (NS3R). However, a homology study of GBV-C and HGV genomes revealed that the 5' untranslated region (5'UTR) was more conserved than NS3R.

METHODS

We attempted to detect GBV-C/HGV using PCR primers corresponding to the 5' UTR, and compared its incidence to that derived from NS3R primers. Furthermore, PCR products amplified using the 5' UTR primers were sequenced and subjected to phylogenetic analysis.

RESULTS

In patients with chronic hepatitis C, the prevalence of GBV-C/HGV by PCR with the NS3R and 5' UTR primers was 5.1% (4/78) and 17.9% (14/78), respectively, and in patients on hemodialysis, it was 0% (0/81) and 5.9% (5/85), respectively. We could not detect GBV-C/HGV in patients with non-A-C liver disease. The incidence of GBV-C/HGV by 5' UTR primers was higher than by NS3R primers. After DNA sequencing at 5' UTR, phylogenetic analysis showed two types of GBV-C/HGV, Jap and HGV types.

CONCLUSION

5' UTR primers proved highly sensitive for detection of GBV-C/HGV and were superior to the NS3R primers.

摘要

目的/背景：许多关于新型肝炎病毒的流行病学研究，包括丙型肝炎病毒（GBV-C）和庚型肝炎病毒（HGV），都使用了针对第三个非结构区域（NS3R）设计的聚合酶链反应（PCR）引物。然而，对GBV-C和HGV基因组的同源性研究表明，5'非翻译区（5'UTR）比NS3R更保守。

方法

我们尝试使用对应于5'UTR的PCR引物检测GBV-C/HGV，并将其发生率与NS3R引物的检测结果进行比较。此外，对使用5'UTR引物扩增的PCR产物进行测序并进行系统发育分析。

结果

在慢性丙型肝炎患者中，使用NS3R引物和5'UTR引物通过PCR检测GBV-C/HGV的患病率分别为5.1%（4/78）和17.9%（14/78），在血液透析患者中分别为0%（0/81）和5.9%（5/85）。我们在非甲-丙型肝病患者中未检测到GBV-C/HGV。5'UTR引物检测GBV-C/HGV的发生率高于NS3R引物。在对5'UTR进行DNA测序后，系统发育分析显示GBV-C/HGV有两种类型，即日本型和HGV型。