Klein G, Reymond J L
Department of Chemistry & Biochemistry, University of Bern, Switzerland.
Bioorg Med Chem Lett. 1998 May 5;8(9):1113-6. doi: 10.1016/s0960-894x(98)00165-6.
3-hydroxybutyl umbelliferyl ethers (R)-1 and (S)-1 are fluorogenic substrates for alcohol dehydrogenases. Their oxidation forms ketone 2, which undergoes beta-elimination of umbelliferone under catalysis by bovine serum albumin, leading to a > 20-fold fluorescence increase at lambda em = 460 +/- 20 nm (lambda ex = 360 +/- 20 nm). Enantioselectivity is determined in two separate tests with each enantiomeric substrate.
3-羟基丁基伞形花内酯醚（R）-1和（S）-1是醇脱氢酶的荧光底物。它们氧化生成酮2，在牛血清白蛋白催化下，酮2发生伞形花内酯的β-消除反应，导致在发射波长λem = 460 ± 20 nm（激发波长λex = 360 ± 20 nm）处荧光增强20倍以上。对映选择性通过对每种对映体底物进行两次单独测试来确定。
