Haneda K, Inazu T, Mizuno M, Iguchi R, Yamamoto K, Kumagai H, Aimoto S, Suzuki H, Noda T
Noguchi Institute, Tokyo, Japan.
Bioorg Med Chem Lett. 1998 Jun 2;8(11):1303-6. doi: 10.1016/s0960-894x(98)00209-1.
Eel calcitonin derivatives containing various N-linked oligosaccharides were chemo-enzymatically synthesized by the transglycosylation reaction of Mucor hiemalis endo-beta-N-acetylglucosaminidase (Endo-M) to a glycosylated calcitonin derivative [Asn(GlcNac)3]-CT in which N-acetyl-D-glycosamine (GlcNAc) is attached to the L-asparagine (Asn) residue of the peptide.
通过毛霉内切β-N-乙酰氨基葡萄糖苷酶（Endo-M）与糖基化降钙素衍生物[Asn(GlcNac)3]-CT进行转糖基化反应，化学酶法合成了含有各种N-连接寡糖的鳗鱼降钙素衍生物，其中N-乙酰-D-葡糖胺（GlcNAc）连接到该肽的L-天冬酰胺（Asn）残基上。
