Quirós E, Piédrola G, Maroto C
Department of Microbiology, Faculty of Medicine, University of Granada, Spain.
Microbios. 1998;95(381):125-30.
The identification of specific genomic sequences of GB viruses (GBV) has made it possible to utilize the polymerase chain reaction (PCR) for evaluation of the viraemia. Several studies have demonstrated the RNA-GBV presence in sera from different patients amplifying several portions of the virus. In this investigation the PCR results when different regions of GBV (NS3, UTR and putative CORE and E1) were amplified in the same sample. In 245 samples studied there were two (0.8%) discordant results and the NS3 primer showed the greatest sensitivity. The lowest percentage of positivity was obtained with CORE-E1 primers. These results could be because the nucleocapside/E1 region was extremely variable in length and sequences, although degenerated primers and probes were used. Discordances were attributable to laboratory errors, variability in the viral genome, the presence of primer inhibitors in samples or a low viral load.
GB病毒（GBV）特定基因组序列的鉴定使得利用聚合酶链反应（PCR）评估病毒血症成为可能。多项研究已证实在不同患者的血清中存在RNA-GBV，这些研究对病毒的多个部分进行了扩增。在本研究中，对同一样本中GBV的不同区域（NS3、UTR以及假定的CORE和E1）进行扩增时的PCR结果。在所研究的245个样本中，有两个（0.8%）结果不一致，且NS3引物显示出最高的敏感性。使用CORE-E1引物时阳性率最低。这些结果可能是因为核衣壳/E1区域在长度和序列上极具变异性，尽管使用了简并引物和探针。结果不一致归因于实验室误差、病毒基因组的变异性、样本中引物抑制剂的存在或病毒载量低。
