Aintablian N, Walpita P, Sawyer M H
University of California, Children's Hospital and Health Center, San Diego, USA.
Infect Control Hosp Epidemiol. 1998 Dec;19(12):918-23. doi: 10.1086/647764.
To evaluate the distribution of Bordetella pertussis and respiratory syncytial virus (RSV) in the hospital setting.
Air samples were collected using filters in the hospital rooms of 12 children with pertussis and 27 children with RSV infection. Material eluted from these filters was subjected to RSV- and B pertussis-specific polymerase chain reaction (PCR) amplification.
Patients were hospitalized in private rooms in one of two referral centers, a university teaching hospital and a university-affiliated private children's hospital.
12 children (16 days-3 years of age) with documented pertussis infection and 27 patients (10 days-7 years of age) with documented RSV infection.
B pertussis DNA was detected in 7 (58%) of 12 rooms housing pertussis patients and in 16 (25%) of 63 total samples. B pertussis DNA was detected as far as 4 m away from the patient's bedside. The detection of B pertussis DNA in air samples did not change over the short duration of hospitalization. RSV RNA was detected in 17 (63%) of 27 rooms housing RSV-infected patients and in 32 (22%) of 143 total samples. RSV RNA was detected at distances as far as 7 m from the patient's bedside and for up to 7 days of hospitalization.
Using PCR-based detection methods, B pertussis DNA and RSV RNA both can be detected in air samples from the hospital rooms of infected patients. Both can be detected at large distances from a patient's bedside in a minority of cases. These detection methods are suitable for further studies of control measures used to contain nosocomial infections caused by both B pertussis and RSV.
评估百日咳博德特氏菌和呼吸道合胞病毒（RSV）在医院环境中的分布情况。
在12例百日咳患儿和27例RSV感染患儿的病房中，使用过滤器采集空气样本。从这些过滤器上洗脱的物质进行RSV和百日咳博德特氏菌特异性聚合酶链反应（PCR）扩增。
患者在两个转诊中心之一的单人病房住院，这两个转诊中心分别是一所大学教学医院和一所大学附属私立儿童医院。
12例确诊百日咳感染的儿童（年龄16天至3岁）和27例确诊RSV感染的患者（年龄10天至7岁）。
在12例百日咳患者的病房中有7间（58%）检测到百日咳博德特氏菌DNA，在63份总样本中有16份（25%）检测到。在距离患者床边4米远处检测到百日咳博德特氏菌DNA。在住院的短时间内，空气样本中百日咳博德特氏菌DNA的检测结果没有变化。在27例RSV感染患者的病房中有17间（63%）检测到RSV RNA，在143份总样本中有32份（22%）检测到。在距离患者床边7米远处以及住院长达7天的时间内均检测到RSV RNA。
使用基于PCR的检测方法，在感染患者病房的空气样本中均可检测到百日咳博德特氏菌DNA和RSV RNA。在少数情况下，在距离患者床边较远处均可检测到这两种病原体。这些检测方法适用于进一步研究控制由百日咳博德特氏菌和RSV引起的医院感染的控制措施。
