Nakazumi H, Sasano H, Iino K, Ohashi Y, Orikasa S
Department of Urology, Tohoku University School of Medicine, Sendai, Japan.
Mod Pathol. 1998 Dec;11(12):1165-70.
Recent immunohistochemical analysis of cell cycle-related proteins such as p27, a cell cycle inhibitory protein, and Ki-67, a proliferation marker, indicated their possible values in predicting the biologic behavior of various human neoplasms. In this study, we performed an immunohistochemical analysis of p27 and Ki-67 in 42 adrenocortical neoplasms (12 adrenocortical carcinomas, 24 adrenocortical adenomas) and 6 normal adrenal glands to evaluate their possible values in diagnosing adrenocortical malignancy and in predicting the biologic behavior of carcinomas. We detected Ki-67 and p27 immunoreactivity in the nuclei of all of our cases, and we observed a significant negative correlation (r = -0.572, P < .001) between the p27 and Ki-67 labeling indexes (LIs). The LIs of p27 and Ki-67 were 61.7+/-2.6 and 0.28+/-0.08 in the normal adrenal cortex and 59.4+/-6.5 and 0.33+/-0.11 in the adenomas, respectively, with no significant differences between the LIs of the adenomas and normal adrenals. The LIs of p27 and Ki-67 in the carcinomas were 48.9+/-7.5 and 630+/-6.21, respectively. The LI of p27 in the carcinomas was significantly lower than that in the adenomas. The LI of Ki-67 in the carcinomas was significantly higher than that in the adenomas (P < .01). Among carcinoma cases, the Ki-67 LI in living cases tended to be lower than that in deceased cases, and the p27 LI in living cases tended to be higher than that in deceased cases, but these differences did not reach statistical significance. These results indicated that decreased p27 protein expression might cause increased cell proliferation in adrenocortical carcinoma cells in combination with other positive and/or negative regulators of the cell cycle. These results also suggested that immunohistochemical analysis of p27 and Ki-67 might be useful in distinguishing between adrenocortical adenoma and carcinoma
最近对细胞周期相关蛋白的免疫组化分析表明，诸如细胞周期抑制蛋白p27和增殖标志物Ki-67等蛋白在预测各种人类肿瘤的生物学行为方面可能具有价值。在本研究中，我们对42例肾上腺皮质肿瘤（12例肾上腺皮质癌、24例肾上腺皮质腺瘤）和6个正常肾上腺进行了p27和Ki-67的免疫组化分析，以评估它们在诊断肾上腺皮质恶性肿瘤及预测癌生物学行为方面的可能价值。我们检测到所有病例细胞核中的Ki-67和p27免疫反应性，并且观察到p27和Ki-67标记指数（LI）之间存在显著负相关（r = -0.572，P <.001）。正常肾上腺皮质中p27和Ki-67的LI分别为61.7±2.6和0.28±0.08，腺瘤中分别为59.4±6.5和0.33±0.11，腺瘤与正常肾上腺的LI之间无显著差异。癌中p27和Ki-67的LI分别为48.9±7.5和63.0±6.21。癌中p27的LI显著低于腺瘤。癌中Ki-67的LI显著高于腺瘤（P <.01）。在癌病例中，存活病例的Ki-6标记指数往往低于死亡病例，存活病例的p27标记指数往往高于死亡病例，但这些差异未达到统计学意义。这些结果表明，p27蛋白表达降低可能与细胞周期的其他正性和/或负性调节因子共同导致肾上腺皮质癌细胞增殖增加。这些结果还表明，p27和Ki-67的免疫组化分析可能有助于区分肾上腺皮质腺瘤和癌
