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1
The alpha subunit of toluene dioxygenase from Pseudomonas putida F1 can accept electrons from reduced FerredoxinTOL but is catalytically inactive in the absence of the beta subunit.来自恶臭假单胞菌F1的甲苯双加氧酶的α亚基可以从还原型铁氧还蛋白TOL接受电子，但在没有β亚基的情况下催化无活性。
Appl Environ Microbiol. 1999 Jan;65(1):315-8. doi: 10.1128/AEM.65.1.315-318.1999.
2
Purification and properties of ferredoxinTOL. A component of toluene dioxygenase from Pseudomonas putida F1.铁氧化还原蛋白TOL的纯化及性质。恶臭假单胞菌F1甲苯双加氧酶的一个组分。
J Biol Chem. 1985 Feb 25;260(4):2355-63.
3
Site-directed mutagenesis of conserved amino acids in the alpha subunit of toluene dioxygenase: potential mononuclear non-heme iron coordination sites.甲苯二加氧酶α亚基中保守氨基酸的定点诱变：潜在的单核非血红素铁配位位点
J Bacteriol. 1996 Jun;178(11):3133-9. doi: 10.1128/jb.178.11.3133-3139.1996.
4
Subtle difference between benzene and toluene dioxygenases of Pseudomonas putida.恶臭假单胞菌苯双加氧酶和甲苯双加氧酶之间的细微差异。
Appl Environ Microbiol. 2005 Mar;71(3):1570-80. doi: 10.1128/AEM.71.3.1570-1580.2005.
5
Construction of hybrid biphenyl (bph) and toluene (tod) genes for functional analysis of aromatic ring dioxygenases.构建用于芳香环双加氧酶功能分析的杂合联苯（bph）和甲苯（tod）基因。
Gene. 1994 Jan 28;138(1-2):27-33. doi: 10.1016/0378-1119(94)90779-x.
6
Isolation and characterization of Pseudomonas putida PpF1 mutants defective in the toluene dioxygenase enzyme system.恶臭假单胞菌PpF1甲苯双加氧酶酶系统缺陷型突变体的分离与鉴定
J Bacteriol. 1984 Dec;160(3):1003-9. doi: 10.1128/jb.160.3.1003-1009.1984.
7
Evaluation of aromatic hydrocarbon decomposition catalyzed by the dioxygenase system and substitution of ferredoxin and ferredoxin reductase.评价双加氧酶系统催化的芳烃分解作用和铁氧还蛋白及铁氧还蛋白还原酶的取代作用。
Environ Sci Pollut Res Int. 2019 Nov;26(33):34047-34057. doi: 10.1007/s11356-018-3200-y. Epub 2018 Sep 23.
8
A novel -2Fe-2S- ferredoxin from Pseudomonas putida mt2 promotes the reductive reactivation of catechol 2,3-dioxygenase.来自恶臭假单胞菌mt2的一种新型-2Fe-2S-铁氧还蛋白促进儿茶酚2,3-双加氧酶的还原再活化。
J Biol Chem. 1998 Apr 17;273(16):9622-9. doi: 10.1074/jbc.273.16.9622.
9
Benzoate 1,2-dioxygenase from Pseudomonas putida: single turnover kinetics and regulation of a two-component Rieske dioxygenase.来自恶臭假单胞菌的苯甲酸1,2-双加氧酶：双组分赖氏双加氧酶的单周转动力学及调控
Biochemistry. 2002 Jul 30;41(30):9611-26. doi: 10.1021/bi025912n.
10
Suppression of electron transfer to dioxygen by charge transfer and electron transfer complexes in the FAD-dependent reductase component of toluene dioxygenase.抑制电子转移到双氧水中的电子转移和电子转移复合物在甲苯双加氧酶的黄素腺嘌呤二核苷酸（FAD）依赖性还原酶组分中。
J Biol Chem. 2012 Nov 2;287(45):38338-46. doi: 10.1074/jbc.M112.374918. Epub 2012 Sep 19.

引用本文的文献

1
Engineering Rieske oxygenase activity one piece at a time.一次一个片段地构建 Rieske 氧合酶活性。
Curr Opin Chem Biol. 2023 Feb;72:102227. doi: 10.1016/j.cbpa.2022.102227. Epub 2022 Nov 18.
2
The α- and β-Subunit Boundary at the Stem of the Mushroom-Like αβ-Type Oxygenase Component of Rieske Non-Heme Iron Oxygenases Is the Rieske-Type Ferredoxin-Binding Site.蘑菇状 αβ-型双氧酶的 Rieske 非血红素铁加氧酶的 α-和 β-亚基结合界面是 Rieske 型铁氧还蛋白结合位点。
Appl Environ Microbiol. 2022 Aug 9;88(15):e0083522. doi: 10.1128/aem.00835-22. Epub 2022 Jul 13.
3
Structural Insights into Dihydroxylation of Terephthalate, a Product of Polyethylene Terephthalate Degradation.对聚对苯二甲酸乙二醇酯降解产物对苯二甲酸二羟化反应的结构研究。
J Bacteriol. 2022 Mar 15;204(3):e0054321. doi: 10.1128/JB.00543-21. Epub 2022 Jan 10.
4
Evaluation of aromatic hydrocarbon decomposition catalyzed by the dioxygenase system and substitution of ferredoxin and ferredoxin reductase.评价双加氧酶系统催化的芳烃分解作用和铁氧还蛋白及铁氧还蛋白还原酶的取代作用。
Environ Sci Pollut Res Int. 2019 Nov;26(33):34047-34057. doi: 10.1007/s11356-018-3200-y. Epub 2018 Sep 23.
5
3-Ketosteroid 9α-hydroxylase enzymes: Rieske non-heme monooxygenases essential for bacterial steroid degradation.3-酮甾体9α-羟化酶：细菌甾体降解所必需的 Rieske 非血红素单加氧酶。
Antonie Van Leeuwenhoek. 2014 Jul;106(1):157-72. doi: 10.1007/s10482-014-0188-2. Epub 2014 May 21.
6
Structures of the multicomponent Rieske non-heme iron toluene 2,3-dioxygenase enzyme system.多组分赖氏非血红素铁甲苯2,3-双加氧酶酶系统的结构
Acta Crystallogr D Biol Crystallogr. 2009 Jan;65(Pt 1):24-33. doi: 10.1107/S0907444908036524. Epub 2008 Dec 18.
7
Similar enzymes, different structures: phthalate dioxygenase is an alpha3alpha3 stacked hexamer, not an alpha3beta3 trimer like "normal" Rieske oxygenases.相似的酶，不同的结构：邻苯二甲酸双加氧酶是一种α3α3堆积的六聚体，而非像“正常”里氏氧化酶那样的α3β3三聚体。
Arch Biochem Biophys. 2007 Oct 1;466(1):31-9. doi: 10.1016/j.abb.2007.07.001. Epub 2007 Jul 14.
8
Characterization of hybrid toluate and benzoate dioxygenases.混合甲苯酸盐和苯甲酸盐双加氧酶的特性分析。
J Bacteriol. 2003 Sep;185(18):5333-41. doi: 10.1128/JB.185.18.5333-5341.2003.
9
The role of active-site residues in naphthalene dioxygenase.活性位点残基在萘双加氧酶中的作用。
J Ind Microbiol Biotechnol. 2003 May;30(5):271-8. doi: 10.1007/s10295-003-0043-3. Epub 2003 Apr 15.
10
Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate.甲苯双加氧酶的实验室进化以接受4-甲基吡啶作为底物。
Appl Environ Microbiol. 2001 Sep;67(9):3882-7. doi: 10.1128/AEM.67.9.3882-3887.2001.

本文引用的文献

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Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase.芳香环羟基化双加氧酶-萘1,2-双加氧酶的结构
Structure. 1998 May 15;6(5):571-86. doi: 10.1016/s0969-2126(98)00059-8.
2
Substrate specificities of hybrid naphthalene and 2,4-dinitrotoluene dioxygenase enzyme systems.杂交萘和2,4-二硝基甲苯双加氧酶系统的底物特异性
J Bacteriol. 1998 May;180(9):2337-44. doi: 10.1128/JB.180.9.2337-2344.1998.
3
Identification and modification of biphenyl dioxygenase sequences that determine the specificity of polychlorinated biphenyl degradation.确定多氯联苯降解特异性的联苯双加氧酶序列的鉴定与修饰。
Appl Environ Microbiol. 1997 Aug;63(8):3096-103. doi: 10.1128/aem.63.8.3096-3103.1997.
4
Functional analyses of a variety of chimeric dioxygenases constructed from two biphenyl dioxygenases that are similar structurally but different functionally.对由两种结构相似但功能不同的联苯双加氧酶构建的多种嵌合双加氧酶进行功能分析。
J Bacteriol. 1997 Jun;179(12):3936-43. doi: 10.1128/jb.179.12.3936-3943.1997.
5
Structural studies on the catalytic component of benzene dioxygenase from Pseudomonas putida.
Biochem Soc Trans. 1997 Feb;25(1):90-5. doi: 10.1042/bst0250090.
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Structure-function analysis of the bacterial aromatic ring-hydroxylating dioxygenases.细菌芳香环羟化双加氧酶的结构-功能分析
Adv Microb Physiol. 1997;38:47-84. doi: 10.1016/s0065-2911(08)60155-1.
7
Rapid purification of the oxygenase component of toluene dioxygenase from a polyol-responsive monoclonal antibody.从一种多元醇反应性单克隆抗体中快速纯化甲苯双加氧酶的加氧酶组分。
Appl Environ Microbiol. 1996 Jun;62(6):2133-7. doi: 10.1128/aem.62.6.2133-2137.1996.
8
Site-directed mutagenesis of conserved amino acids in the alpha subunit of toluene dioxygenase: potential mononuclear non-heme iron coordination sites.甲苯二加氧酶α亚基中保守氨基酸的定点诱变：潜在的单核非血红素铁配位位点
J Bacteriol. 1996 Jun;178(11):3133-9. doi: 10.1128/jb.178.11.3133-3139.1996.
9
Characterization of active recombinant his-tagged oxygenase component of Comamonas testosteroni B-356 biphenyl dioxygenase.睾丸酮丛毛单胞菌B-356联苯双加氧酶活性重组组氨酸标签加氧酶组分的表征
J Biol Chem. 1996 Apr 5;271(14):8152-6. doi: 10.1074/jbc.271.14.8152.
10
Coordination of the Rieske-type [2Fe-2S] cluster of the terminal iron-sulfur protein of Pseudomonas putida benzene 1,2-dioxygenase, studied by one- and two-dimensional electron spin-echo envelope modulation spectroscopy.通过一维和二维电子自旋回波包络调制光谱研究恶臭假单胞菌苯1,2-双加氧酶末端铁硫蛋白的 Rieske 型 [2Fe-2S] 簇的配位情况。
Biochemistry. 1995 Dec 26;34(51):16533-42. doi: 10.1021/bi00051a001.

来自恶臭假单胞菌F1的甲苯双加氧酶的α亚基可以从还原型铁氧还蛋白TOL接受电子，但在没有β亚基的情况下催化无活性。

The alpha subunit of toluene dioxygenase from Pseudomonas putida F1 can accept electrons from reduced FerredoxinTOL but is catalytically inactive in the absence of the beta subunit.

作者信息

Jiang H, Parales R E, Gibson D T

机构信息

Department of Microbiology and Center for Biocatalysis and Bioprocessing, The University of Iowa, Iowa City, Iowa 52242, USA.

出版信息

Appl Environ Microbiol. 1999 Jan;65(1):315-8. doi: 10.1128/AEM.65.1.315-318.1999.

DOI:10.1128/AEM.65.1.315-318.1999
PMID:9872799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC91022/
Abstract

The oxygenase component of toluene dioxygenase from Pseudomonas putida F1 is an iron-sulfur protein (ISPTOL) consisting of alpha (TodC1) and beta (TodC2) subunits. Purified TodC1 gave absorbance and electron paramagnetic resonance spectra identical to those given by purified ISPTOL. TodC1 was reduced by NADH and catalytic amounts of ReductaseTOL and FerredoxinTOL. Reduced TodC1 did not oxidize toluene, and catalysis was strictly dependent on the presence of purified TodC2.

摘要

恶臭假单胞菌F1甲苯双加氧酶的加氧酶组分是一种铁硫蛋白（ISPTOL），由α（TodC1）和β（TodC2）亚基组成。纯化后的TodC1的吸光度和电子顺磁共振光谱与纯化后的ISPTOL相同。TodC1可被NADH以及催化量的还原酶TOL和铁氧还蛋白TOL还原。还原后的TodC1不能氧化甲苯，催化作用严格依赖于纯化后的TodC2的存在。