Eckhardt F, Meyerhans A
Institute of Medical Microbiology and Hygiene, Department of Virology, University of the Saarland, Homburg, Germany.
Neuroreport. 1998 Dec 1;9(17):3975-9. doi: 10.1097/00001756-199812010-00038.
The semaphorin/collapsin family of proteins comprises molecules thought to be important for the guidance of growing axons. All members of this family, which includes both secreted and cell-surface molecules, share a conserved domain of approximately 500 amino acids. Here, we report the cloning of a novel murine semaphorin, termed M-sema IV. It displays 96.3% identity to the human semaphorin H-sema IV and is therefore likely to be the respective murine homologue. In addition, an isoform was identified, which contains an additional 31 amino acids in the semaphorin domain. M-sema IV appears to be expressed ubiquitously in adulthood. During embryogenesis, in situ hybridization revealed M-sema IV expression in subregions of the central nervous system and various other tissues like skin, kidney, lung and intestine.
信号素/塌陷蛋白家族的蛋白质包含被认为对生长中的轴突导向很重要的分子。该家族的所有成员，包括分泌型和细胞表面分子，都共享一个约500个氨基酸的保守结构域。在此，我们报告了一种新型小鼠信号素的克隆，称为M-信号素IV。它与人信号素H-信号素IV具有96.3%的同一性，因此很可能是各自的小鼠同源物。此外，还鉴定出一种异构体，其在信号素结构域中含有另外31个氨基酸。M-信号素IV在成年期似乎普遍表达。在胚胎发育过程中，原位杂交显示M-信号素IV在中枢神经系统的亚区域以及皮肤、肾脏、肺和肠道等各种其他组织中表达。
