Simmons P A, Tomlinson A, Seal D V
Southern California College of Optometry, Fullerton 92831, USA.
Optom Vis Sci. 1998 Dec;75(12):860-6. doi: 10.1097/00006324-199812000-00007.
The effect of the previous coating of a contact lens surface with Pseudomonas biofilm on adsorption of Acanthamoeba onto four types of hydrogel materials was investigated.
Hydrogel contact lens quarters from each of the four FDA groups of hydrogel materials were incubated for at least 12 h in a suspension of 10(7)/ml of Pseudomonas aeruginosa (ATCC 27853) to coat their surfaces with biofilm. After rinsing, the lenses were incubated for 90 min in 5 x 10(5)/ml of Acanthamoeba castellanii trophozoites. New, uncoated lens quarters were incubated in the Acanthamoeba suspension as controls. After rinsing, all adsorbed trophozoites on one surface of each lens quarter were counted by direct light microscopy. Adsorption was expressed as numbers of amoebae per square centimeter of lens surface, and nonparametric data analysis was performed.
Acanthamoeba adsorption to new, uncoated lenses was greater for ionic materials (groups 3 and 4) than for the nonionic materials (groups 1 and 2). Pseudomonas biofilm increased adsorption on all four lens types.
Pseudomonas biofilm enhanced adsorption of Acanthamoeba on all lens types studied, but the adsorption to nonionic materials was significantly less. This suggests that all lens wearers may be at increased risk for Acanthamoeba infection if lenses are previously contaminated with bacterial biofilm, but this risk may be reduced by the use of certain lens types (low water content, nonionic materials).
研究隐形眼镜表面预先形成的铜绿假单胞菌生物膜对棘阿米巴在四种水凝胶材料上吸附的影响。
从美国食品药品监督管理局（FDA）划分的四类水凝胶材料中各取隐形眼镜四分之一片，置于浓度为10(7)/ml的铜绿假单胞菌（ATCC 27853）悬液中孵育至少12小时，使其表面形成生物膜。冲洗后，将镜片置于浓度为5×10(5)/ml的卡氏棘阿米巴滋养体悬液中孵育90分钟。取未涂覆的新镜片四分之一片作为对照，置于棘阿米巴悬液中孵育。冲洗后，通过直接光学显微镜对每片镜片四分之一片一个表面上吸附的所有滋养体进行计数。吸附量以每平方厘米镜片表面的阿米巴数量表示，并进行非参数数据分析。
离子型材料（第3组和第4组）的新的未涂覆镜片对棘阿米巴的吸附量高于非离子型材料（第1组和第2组）。铜绿假单胞菌生物膜增加了所有四种镜片类型上的吸附量。
铜绿假单胞菌生物膜增强了棘阿米巴在所有研究的镜片类型上的吸附，但对非离子型材料的吸附明显较少。这表明，如果镜片先前被细菌生物膜污染，所有隐形眼镜佩戴者感染棘阿米巴的风险可能会增加，但使用某些镜片类型（低含水量、非离子型材料）可能会降低这种风险。
