Gorlin A I, Gabriel M M, Wilson L A, Ahearn D G
Department of Biology, Georgia State University, Atlanta, USA.
Arch Ophthalmol. 1996 May;114(5):576-80. doi: 10.1001/archopht.1996.01100130568013.
To determine the effect of Pseudomonas aeruginosa and Staphylococcus epidermidis on the binding of Acanthamoeba species to hydrogel lenses.
Cells of amebae and bacteria were incubated with different types of hydrogel lenses. Densities of amebae that were bound to the lenses after rinsing were determined from direct counts with a cell detachment procedure and from scintillation counts of cells, which were radiolabeled with tritiated leucine.
With both methods, amebae showed significantly increased binding to hydrogel lenses with attached P aeruginosa. The numbers of amebae that were retained on lenses with attached S epidermidis were not significantly different from those that were retained on lenses without bacteria. The binding of amebae to unworn hydrogel lenses, in contrast to the irreversible adherence of P aeruginosa, was tenuous.
The binding of Acanthamoeba species to unworn hydrogel lenses was tenuous and appeared to be related to water content, surface tensions, and ionic charge. The presence of adhered P aeruginosa on the hydrogel lenses facilitated the binding of Acanthamoeba species. The cocontamination of lens systems with bacteria (eg, P aeruginosa) may be a prime factor in the development of amebic keratitis.
确定铜绿假单胞菌和表皮葡萄球菌对棘阿米巴属物种与水凝胶镜片结合的影响。
将阿米巴细胞和细菌细胞与不同类型的水凝胶镜片一起孵育。冲洗后结合在镜片上的阿米巴密度通过细胞分离程序直接计数以及用氚标记亮氨酸进行放射性标记的细胞闪烁计数来确定。
两种方法均显示，阿米巴与附着有铜绿假单胞菌的水凝胶镜片的结合显著增加。附着有表皮葡萄球菌的镜片上保留的阿米巴数量与没有细菌的镜片上保留的数量没有显著差异。与铜绿假单胞菌的不可逆黏附相反，阿米巴与未使用过的水凝胶镜片的结合很微弱。
棘阿米巴属物种与未使用过的水凝胶镜片的结合很微弱，似乎与含水量、表面张力和离子电荷有关。水凝胶镜片上附着的铜绿假单胞菌促进了棘阿米巴属物种的结合。镜片系统被细菌（如铜绿假单胞菌）共污染可能是阿米巴性角膜炎发生的主要因素。
