Morrow C J, Markham J F, Whithear K G
School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Avian Dis. 1998 Oct-Dec;42(4):667-70.
An Australian field isolate of Mycoplasma synoviae (MS), 89079/7NS, was exposed to the mutagen N-nitro-N'-methyl-N-nitrosoguanidine. Fifteen clones from the exposed culture were characterized for temperature sensitivity. Four clones labelled B, D, G, and H were temperature sensitive and were further characterized for their ability to colonize chickens and elicit an immune response. Serum antibody responses to MS were detected 3 wk after infection, by eyedrop, in 10 of 10 birds inoculated with 86079/7NS and clones B and G and in 9 of 10 birds inoculated with clone H. No MS antibody response was observed in any bird inoculated with clone D. MS was recovered from the upper trachea of 10 of 10 birds inoculated with clones B, G, and H at 2 wk after infection. No MS was isolated from birds inoculated with clone D. Clone H, designated MS-H, was selected as a potential vaccine candidate.
一株澳大利亚滑膜支原体（MS）野外分离株89079/7NS，用诱变剂N-硝基-N'-甲基-N-亚硝基胍处理。对来自处理后培养物的15个克隆进行温度敏感性鉴定。标记为B、D、G和H的4个克隆对温度敏感，并进一步鉴定它们在鸡体内定殖和引发免疫反应的能力。感染3周后，通过滴眼法检测到，接种86079/7NS以及克隆B和G的10只鸡中有10只、接种克隆H的10只鸡中有9只出现了针对MS的血清抗体反应。接种克隆D的任何一只鸡均未观察到MS抗体反应。感染2周后，接种克隆B、G和H的10只鸡中有10只的上呼吸道中分离出了MS。接种克隆D的鸡未分离到MS。克隆H，命名为MS-H，被选为潜在的疫苗候选株。
