Achen M, Morishita T Y, Ley E C
Department of Veterinary Preventive Medicine, Ohio State University, Columbus 43210, USA.
Avian Dis. 1998 Oct-Dec;42(4):732-7.
Poultry are considered to be the primary reservoirs of Campylobacter jejuni for humans. Campylobacter jejuni can colonize the poultry intestinal tract and its subsequent shedding can result in environmental contamination, resulting in an increased risk of infection for the rest of the flock. At present, there is no information on the daily shedding pattern of C. jejuni in broiler chickens. Thus, the purpose of this study was to determine the daily shedding pattern of C. jejuni in broiler chickens and to correlate intestinal colonization with fecal shedding, which would aid in the development of intervention strategies such as the use of competitive exclusion products at 1 day of age. Twenty-four broiler chicks were orally inoculated with 1.6 x 10(7) colony-forming units of C. jejuni, and the reisolation rate of the organism was determined daily from day 1 to day 43. Fifty percent and 70% of the chicks were shedding C. jejuni within 24 and 48 hr postinoculation, respectively. The group collectively reached a peak excretion on days 13-19 postinoculation. There was a steady decline in fecal shedding after the third week. By market age, on day 43, only 37.5% (9/24) of the birds were shedding C. jejuni in their feces. Throughout the sampling period from days 1 to 43, a cyclic pattern of shedding was observed in individual birds. Individual birds excreted C. jejuni on an average of 25 out of 43 days. The C. jejuni isolate failed to colonize 16.6% (4/24) of the birds. A small percentage of the birds, 12.5% (3/24), were observed to be chronic shedders. Enumeration of C. jejuni in the crop, jejunum, and cecum on day 43 revealed that the cecum was the major colonization site, and 15 out of the 24 birds carried C. jejuni in their intestinal tract.
家禽被认为是人类空肠弯曲菌的主要储存宿主。空肠弯曲菌可在禽肠道内定植，其随后的排泄会导致环境污染，从而增加鸡群其他个体的感染风险。目前，关于肉鸡中空肠弯曲菌的每日排泄模式尚无相关信息。因此，本研究的目的是确定肉鸡中空肠弯曲菌的每日排泄模式，并将肠道定植与粪便排泄相关联，这将有助于制定干预策略，例如在1日龄时使用竞争排斥产品。24只肉鸡雏鸡经口接种1.6×10⁷空肠弯曲菌菌落形成单位，从第1天至第43天每天测定该菌的重新分离率。分别有50%和70%的雏鸡在接种后24小时和48小时内排出空肠弯曲菌。该组在接种后第13 - 19天集体达到排泄高峰。第三周后粪便排泄量稳步下降。到上市日即第43天，只有37.5%（9/24）的鸡粪便中排出空肠弯曲菌。在第1天至第43天的整个采样期间，在个体鸡中观察到排泄的循环模式。个体鸡在43天中有25天平均排出空肠弯曲菌。空肠弯曲菌分离株未能在16.6%（4/24）的鸡中定植。观察到一小部分鸡，即12.5%（3/24），为空肠弯曲菌慢性排泄者。在第43天对嗉囊、空肠和盲肠中的空肠弯曲菌进行计数，结果显示盲肠是主要的定植部位，24只鸡中有15只肠道内携带空肠弯曲菌。
