Davison S, Ziegler A F, Eckroade R J
University of Pennsylvania, School of Veterinary Medicine, Laboratory of Avian Medicine and Pathology, Kennett Square 19348, USA.
Avian Dis. 1998 Oct-Dec;42(4):791-5.
The standard tests used to detect avian influenza (AI) viral infection include virus isolation from tissues of the infected birds and the detection of AI antibody in blood or egg yolk. A new application of an existing human test to rapidly detect the presence of any influenza A virus is now possible. A commercially available antigen-capture enzyme immunoassay (AC-EIA), developed for the detection of influenza A in humans was tested for relative sensitivity and specificity and for speed of use in diagnosing nonpathogenic H7N2 AI in naturally infected poultry. During the recent nonpathogenic H7N2 AI epornitic, the AC-EIA was used for rapid diagnosis and quarantine decisions. Between February and August 1997, 1524 samples from 295 commercial layer, pullet, and broiler flocks were submitted to the Laboratory of Avian Medicine and Pathology, New Bolton Center, for AI virus isolation and testing by AC-EIA. The relative specificity of the AC-EIA was 100% and the relative sensitivity was 79%. We believe that the AC-EIA will be a useful adjunct to standard AI diagnostic tests.
用于检测禽流感（AI）病毒感染的标准测试包括从受感染禽类的组织中分离病毒以及检测血液或蛋黄中的禽流感抗体。现在，一种现有的人类测试方法有了新应用，可以快速检测任何甲型流感病毒的存在。一种为检测人类甲型流感而开发的市售抗原捕获酶免疫测定法（AC-EIA），针对其相对敏感性、特异性以及在诊断自然感染家禽中的非致病性H7N2禽流感时的使用速度进行了测试。在最近的非致病性H7N2禽流感流行期间，AC-EIA被用于快速诊断和检疫决策。1997年2月至8月期间，来自295个商业蛋鸡、小母鸡和肉鸡群的1524份样本被提交到新博尔顿中心禽医学与病理学实验室，用于禽流感病毒分离和AC-EIA检测。AC-EIA的相对特异性为100%，相对敏感性为79%。我们认为，AC-EIA将成为标准禽流感诊断测试的有用辅助手段。
