National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Science, Xiamen University, 422 South Siming Rd., Xiamen, China.
J Virol Methods. 2010 Jul;167(1):100-2. doi: 10.1016/j.jviromet.2010.02.001. Epub 2010 Feb 6.
A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus.
一种新的流感 A 病毒快速诊断检测方法进行了四组实验评估：首先，与一种商业诊断试剂盒针对一组病毒株进行了比较；其次，该试剂盒针对从五个不同宿主物种分离的 40 株流感 A 病毒和 26 株作为对照的其他呼吸道病毒进行了测试；第三，该试剂盒针对从人类和鸡采集的现场标本进行了测试；第四，该试剂盒针对从住院患者中获得的新型大流行流感 A/H1N1 2009 临床标本进行了测试。该检测试剂盒对人类和禽类标本的灵敏度分别为 88%。相应的特异性分别为 99.3%的人类标本和 96.5%的禽类标本。该检测试剂盒可能有助于快速诊断流感 A 病毒。
