生物系统中活性氧物种的检测。
Detection of active oxygen species in biological systems.
作者信息
Nakano M
机构信息
Department of Photon and Free Radical Research, Japan Immunoresearch Laboratories Co., Ltd., Gunma, Japan.
出版信息
Cell Mol Neurobiol. 1998 Dec;18(6):565-79. doi: 10.1023/a:1020621616389.
Abstract
- Cypridina luciferin analogues, 2-methyl-6-(p-methoxyphenyl)-3,7- dihydroimidazo[1,2-a]pyrazin-3-one (MCLD) and 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one(CLA ), react with O2- or 1O2 to emit light in visible region. Such chemiluminescences were used for the detection of O2- or 1O2 in activated leukocyte systems and myeloperoxidase (granulocyte-extract) + Br- + H2O2 systems in vitro. 2. The mechanisms of MCLA (CLA)-dependent luminescence is described in detail. Superoxide generated from sinusoidal cells in acute ethanol intoxication of rats was detected by MCLA-dependent luminescence from the surface of perfused rat liver (organ luminescence). 3. Furthermore, with alive animals, O2- generated in the lung of rats with necrotized pancreatitis and that in the stomach of rats after ischemia/reperfusion were detected by their organ luminescences.
摘要
- 海萤荧光素类似物，2-甲基-6-(对甲氧基苯基)-3,7-二氢咪唑并[1,2-a]吡嗪-3-酮（MCLD）和2-甲基-6-苯基-3,7-二氢咪唑并[1,2-a]吡嗪-3-酮（CLA），与超氧阴离子（O₂⁻）或单线态氧（¹O₂）反应，在可见光区域发光。这种化学发光用于体外检测活化白细胞系统以及髓过氧化物酶（粒细胞提取物）+ Br⁻ + H₂O₂系统中的O₂⁻或¹O₂。2. 详细描述了MCLA（CLA）依赖性发光的机制。通过灌注大鼠肝脏表面的MCLA依赖性发光（器官发光）检测大鼠急性乙醇中毒时窦状细胞产生的超氧化物。3. 此外，在活体动物中，通过器官发光检测坏死性胰腺炎大鼠肺中以及缺血/再灌注后大鼠胃中产生的O₂⁻。
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