Yagi M, Adachi J, Tatsuno Y, Mizuno K
Department of Orthopaedic Surgery, Kobe University School of Medicine, Japan.
Clin Chim Acta. 1998 Nov;278(1):67-74. doi: 10.1016/s0009-8981(98)00156-9.
The nitric oxide profile produced by application of a pneumatic tourniquet was investigated in the plasma of 15 patients. Nitrite (NO2-) and nitrate (NO3-) plasma concentrations were measured simultaneously by high-performance liquid chromatography (HPLC). UV detection using the Griess reaction was done after the reduction of nitrate to nitrite. The plasma nitrate concentration 5 min after reperfusion was significantly higher than the concentrations before ischemia, immediately before reperfusion, and the next day. In contrast, there was no significant difference in the plasma nitrite concentrations before ischemia, immediately before reperfusion, 5 min after reperfusion, and the next day. These findings suggest that the generation of NO is important in ischemic reperfusion injury.
对15例患者的血浆进行研究，以探究应用气动止血带后产生的一氧化氮情况。通过高效液相色谱法（HPLC）同时测定血浆中亚硝酸盐（NO2-）和硝酸盐（NO3-）的浓度。在将硝酸盐还原为亚硝酸盐后，采用格里斯反应进行紫外线检测。再灌注后5分钟时的血浆硝酸盐浓度显著高于缺血前、再灌注前即刻及次日的浓度。相比之下，缺血前、再灌注前即刻、再灌注后5分钟及次日的血浆亚硝酸盐浓度无显著差异。这些研究结果表明，一氧化氮的生成在缺血再灌注损伤中起重要作用。
