Schaffer F L, Soergel M E, Straube D C
Arch Virol. 1976;51(4):263-73. doi: 10.1007/BF01317930.
Influenza A virus, strain WSNH, propagated in bovine, human and chick embryo cell cultures and aerosolized from the cell culture medium, was maximally stable at low relative humidity (RH), minimally stable at mid-range RH, and moderately stable at high RH. Most lots of WSNH virus propagated in embryonated eggs and aerosolized from the allantoic fluid were also least stable at mid-range RH, but two preparations after multiple serial passage in eggs showed equal stability at mid-range and higher RH's. Airborne stability varied from preparation to preparations of virus propagated both in cell culture and embryonal eggs. There was no apparent correlation between airborne stability and protein content of spray fluid above 0.1 mg/ml, but one preparation of lesser protein concentration was extremely unstable at 50 to 80 per cent RH. Polyhydroxy compounds exerted a protective effect on airborne stability.
甲型流感病毒WSNH株，在牛、人及鸡胚细胞培养物中繁殖，并从细胞培养基雾化后，在低相对湿度（RH）下稳定性最高，在中等RH下稳定性最低，在高RH下稳定性中等。大多数在鸡胚中繁殖并从尿囊液雾化的WSNH病毒批次在中等RH下稳定性也最低，但在鸡胚中多次连续传代后的两种制剂在中等及更高RH下显示出相同的稳定性。细胞培养物和鸡胚中繁殖的病毒制剂之间，空气传播稳定性各不相同。当喷雾液蛋白质含量高于0.1mg/ml时，空气传播稳定性与蛋白质含量之间没有明显相关性，但一种蛋白质浓度较低的制剂在50%至80%RH下极不稳定。多羟基化合物对空气传播稳定性有保护作用。
