Tangy F, Ossondo M, Vernant J C, Smadja D, Blétry O, Baglin A C, Ozden S
Unité des Virus Lents, Institut Pasteur, 75274 Paris Cedex 15, France.
J Infect Dis. 1999 Feb;179(2):497-502. doi: 10.1086/314588.
Human T cell leukemia virus type I (HTLV-I) sequences were sought in labial salivary glands of patients with HTLV-I-associated myelopathy or tropical spastic paraparesis and of seropositive neurologically healthy carriers. HTLV-I proviral DNA was found by polymerase chain reaction amplification in DNA extracted from lip biopsies of every patient. Viral RNA was found by in situ hybridization in the acini epithelium, as well as in lymphocytic infiltrates. This observation suggests that HTLV-I expression in labial salivary glands could participate in the inflammatory lesions observed in these patients. Some seronegative patients with Sjögren's syndrome or dryness syndrome were also positive for viral transactivator tax DNA (41% in Martinique and 16% in non-HTLV-I-endemic region). Despite histologic signs of lymphocytic infiltration, no viral expression was found in the labial salivary glands of these patients.
在患有人类嗜T淋巴细胞病毒I型（HTLV-I）相关脊髓病或热带痉挛性截瘫的患者以及血清学阳性的神经功能正常携带者的唇唾液腺中寻找HTLV-I序列。通过聚合酶链反应扩增，在从每位患者的唇部活检提取的DNA中发现了HTLV-I前病毒DNA。通过原位杂交在腺泡上皮以及淋巴细胞浸润中发现了病毒RNA。这一观察结果表明，唇唾液腺中HTLV-I的表达可能参与了这些患者中观察到的炎性病变。一些患有干燥综合征或干燥症的血清学阴性患者的病毒反式激活因子tax DNA也呈阳性（在马提尼克岛为41%，在非HTLV-I流行地区为16%）。尽管有淋巴细胞浸润的组织学迹象，但在这些患者的唇唾液腺中未发现病毒表达。
