Ohyama Y, Nakamura S, Hara H, Shinohara M, Sasaki M, Ikebe-Hiroki A, Mouri T, Tsunawaki S, Abe K, Shirasuna K, Nomoto K
Kyushu University, Fukuoka, Japan.
Arthritis Rheum. 1998 Nov;41(11):1972-8. doi: 10.1002/1529-0131(199811)41:11<1972::AID-ART12>3.0.CO;2-M.
To clarify the involvement of human T lymphotropic virus type I (HTLV-I) in the pathogenesis of Sjogren's syndrome (SS).
In HTLV-I-seropositive patients with SS, HTLV-I proviral DNA in the labial salivary glands (SG) was detected by polymerase chain reaction (PCR) amplification of the extracted cellular DNA, and the localization in the SG was examined by in situ PCR hybridization.
The cellular DNA extracted from the SG contained full HTLV-I proviral DNA, which was present in the nucleus of the infiltrating T cells, but not in either the SG epithelial cells or the acinar cells. Furthermore, the viral loads in the SG were approximately 8 times to 9 x 10(3) times higher than those in the peripheral blood mononuclear cells.
Accumulation of HTLV-I-infected T cells in the SG suggests that HTLV-I likely causes the self-reactive T cells to proliferate, which, as a result, induces SS.
阐明I型人类嗜T淋巴细胞病毒（HTLV-I）在干燥综合征（SS）发病机制中的作用。
在HTLV-I血清学阳性的SS患者中，通过聚合酶链反应（PCR）扩增提取的细胞DNA来检测唇唾液腺（SG）中的HTLV-I前病毒DNA，并通过原位PCR杂交检查其在SG中的定位。
从SG中提取的细胞DNA含有完整的HTLV-I前病毒DNA，其存在于浸润性T细胞核中，但不存在于SG上皮细胞或腺泡细胞中。此外，SG中的病毒载量比外周血单个核细胞中的病毒载量高约8倍至9×10³倍。
SG中HTLV-I感染的T细胞积聚表明HTLV-I可能导致自身反应性T细胞增殖，从而诱发SS。
