Waddington J L, Crow T J
Psychopharmacology (Berl). 1978 Jul 6;58(2):153-5. doi: 10.1007/BF00426898.
Rats were lesioned unilaterally in the medial forebrain bundle with either the catecholamine neurotoxin 6-hydroxydopamine or the indoleamine neurotoxin 5,6-dihydroxytryptamine. Their rotational responses in automated rotameters to a challenge with the dopamine-receptor agonist apomorphine were compared using four different techniques in current use, and by assessment of complete rotation curves using both conventional statistical procedures and elementary computer-derived elements of curvature. The rotational responses of the two groups, characterized neurochemically by identical depletions of striatal dopamine but with a greater depletion of striatal 5-hydroxytryptamine in 5,6-dihydroxytryptamine-lesioned animals, were indistinguishable using each of the four current techniques. Assessment of rotation curves by both methods revealed significant differences between the two groups, characterised by faster onset and offset of the rotational response in 5,6-dihydroxytryptamine-lesioned animals. Some current techniques may implicitly exclude the detection of such time-course differences in rotational behaviour. Assessment of complete rotation curves may best allow valid comparisons between experimental groups.
大鼠单侧内侧前脑束用儿茶酚胺神经毒素6-羟基多巴胺或吲哚胺神经毒素5,6-二羟基色胺进行损伤。使用当前常用的四种不同技术，通过使用传统统计程序和基本计算机衍生的曲率元素评估完整的旋转曲线，比较了它们在自动旋转仪中对多巴胺受体激动剂阿扑吗啡激发的旋转反应。两组的旋转反应在神经化学上的特征是纹状体多巴胺的相同消耗，但在5,6-二羟基色胺损伤的动物中纹状体5-羟色胺的消耗更大，使用目前的四种技术中的每一种都无法区分。通过两种方法评估旋转曲线发现两组之间存在显著差异，其特征是5,6-二羟基色胺损伤的动物中旋转反应的起始和偏移更快。一些当前技术可能会隐含地排除对旋转行为中这种时间过程差异的检测。评估完整的旋转曲线可能最能有效地对实验组进行有效比较。
