Serafino A, Valli M B, Andreola F, Carloni G, Bertolini L
Laboratorio di Microscopia Elettronica e Confocale-Area di Ricerca di Roma Tor Vergata, CNR, Rome.
Res Virol. 1998 Sep-Oct;149(5):299-305. doi: 10.1016/s0923-2516(99)89009-8.
In this study, we analysed by transmission electron microscopy (TEM), sequential details of morphological modifications that accompanied viral morphogenesis in the lymphoblastoid cell line (LCL) TOFE infected in vitro with hepatitis C virus (HCV). As previously reported, we observed virus-like particles (VLPs) in cytoplasmic vesicles mainly located in the perinuclear region of infected cells. In this area, the Golgi apparatus and the endoplasmic reticulum (ER) appeared hyperplastic, remarkably enriched in vesicles and lysosomal structures. Furthermore, only in this perinuclear region, cytopathic-effect(CPE)-like changes seemed to originate, consisting in enlarged cytoplasmic vacuoles filled with degenerative amorphous material containing VLPs. Finally, the complete filling-up of the cytoplasm with these degenerative vacuoles, in addition to cellular lysis displayed by some cells, appeared as the possible terminal pattern of the infectious process. Our data suggest that in vitro HCV-infected TOFE cells undergo typical CPE-like changes that may be connected with virus replication.
在本研究中，我们通过透射电子显微镜（TEM）分析了体外感染丙型肝炎病毒（HCV）的淋巴母细胞样细胞系（LCL）TOFE中伴随病毒形态发生的形态学修饰的连续细节。如先前报道，我们在主要位于受感染细胞核周区域的细胞质囊泡中观察到病毒样颗粒（VLP）。在该区域，高尔基体和内质网（ER）出现增生，囊泡和溶酶体结构显著富集。此外，仅在该核周区域似乎出现了细胞病变效应（CPE）样变化，表现为充满含有VLP的退行性无定形物质的扩大的细胞质空泡。最后，这些退行性空泡完全充满细胞质，再加上一些细胞显示出的细胞裂解，似乎是感染过程的可能终末模式。我们的数据表明，体外HCV感染的TOFE细胞会经历典型的CPE样变化，这可能与病毒复制有关。
