Soares R P, Gontijo N F, Romanha A J, Diotaiuti L, Pereira M H
Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Acta Trop. 1998 Nov 30;71(3):285-91. doi: 10.1016/s0001-706x(98)00065-5.
Rhodnius prolixus interpopulation variability was studied based on a new approach using salivary heme proteins (nitrophorins) electrophoresis in starch gel. We compared salivary proteins profiles of R. prolixus from three different laboratory colonies from Honduras, Venezuela, Brazil and Rhodnius robustus from Venezuela, constructing a UPGMA. The Honduran and Venezuelan populations could not be distinguished from each other, but the Brazilian population was well separated from the others. The high similarity between Honduran and Venezuelan specimens lends support to current theories that the Central American populations of R. prolixus may have been introduced from a Venezuelan origin. The low polymorphism shown by the Honduran specimens is in agreement with a possible founder effect. This new approach also distinguished R. prolixus populations from R. robustus, species with extreme phenotypical similarity.
基于一种新方法，即利用唾液血红素蛋白（亲硝蛋白）在淀粉凝胶中的电泳，对长红猎蝽种群间的变异性进行了研究。我们比较了来自洪都拉斯、委内瑞拉、巴西的三个不同实验室群体的长红猎蝽以及来自委内瑞拉的粗壮猎蝽的唾液蛋白图谱，构建了一个UPGMA（非加权组平均法）。洪都拉斯和委内瑞拉的种群彼此无法区分，但巴西种群与其他种群明显分开。洪都拉斯和委内瑞拉标本之间的高度相似性支持了当前的理论，即中美洲的长红猎蝽种群可能起源于委内瑞拉。洪都拉斯标本显示的低多态性与可能的奠基者效应一致。这种新方法还区分了长红猎蝽种群和粗壮猎蝽种群，这两个物种具有极高的表型相似性。
