Hansen S H, Jensen M E, Bjørnsdottir I
Department of Analytical and Pharmaceutical Chemistry, The Royal Danish School of Pharmacy, Copenhagen.
J Pharm Biomed Anal. 1998 Sep 1;17(6-7):1155-60. doi: 10.1016/s0731-7085(98)00081-8.
The separation of acetylsalicylic acid and three of its metabolites--salicylic acid, salicyluric acid and gentisic acid--is demonstrated in a non-aqueous capillary electrophoresis system with reversed electroosmotic flow. Solvent mixtures of methanol and acetonitrile are used for the electrophoresis media and different electrolytes have been investigated. The flow is reversed by the addition of the polycation hexadimethrine bromide and thus a negative voltage is used. This system provides a fast and effective separation of the four analytes. The separation method was applied to the assay of acetylsalicylic acid and its major metabolites in plasma and urine and the limits of quantification for all of these compounds are about 5 microg ml(-1) in plasma and 25 microg ml(-1) in urine.
在具有反向电渗流的非水毛细管电泳系统中，实现了乙酰水杨酸及其三种代谢物——水杨酸、水杨尿酸和龙胆酸的分离。甲醇和乙腈的混合溶剂用作电泳介质，并对不同的电解质进行了研究。通过添加聚阳离子溴化六甲双铵使流动反向，因此使用负电压。该系统能快速有效地分离这四种分析物。该分离方法应用于血浆和尿液中乙酰水杨酸及其主要代谢物的测定，所有这些化合物在血浆中的定量限约为5 μg ml⁻¹，在尿液中的定量限约为25 μg ml⁻¹。
