Kodjo A, Villard L, Bizet C, Martel J L, Sanchis R, Borges E, Gauthier D, Maurin F, Richard Y
Ecole Nationale Vétérinaire de Lyon, F-69280 Marcy l'Etoile, France.
J Clin Microbiol. 1999 Feb;37(2):380-5. doi: 10.1128/JCM.37.2.380-385.1999.
One hundred thirty-three strains of Pasteurella haemolytica of both biotypes (90 and 43 strains of biotypes A and T, respectively) and almost all the serotypes were subjected to ribotyping, random amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE) analysis for epidemiological purposes. A total of 15 patterns recorded as ribotypes HA to HO were found for the P. haemolytica biotype A strains, with ribotypes HA, HC, and HD being encountered most often (66 strains [74%]); and 20 ribotypes, designated HA' to HT', that were clearly distinct from those observed for biotype A strains were observed for strains of biotype T. RAPD analysis generated a total of 44 (designated Rp1 to Rp44) and 15 (designated Rp1' to Rp 15') unique RAPD patterns for biogroup A and biogroup T, respectively. Analysis of the data indicated that a given combined ribotype-RAPD pattern could be observed for biotype A strains of different serotypes, whatever the zoological or geographic origin, whereas this was not the case for biotype T strains. PFGE appeared to be more efficient in strain discrimination since selected strains from various zoological or geographical origins harboring the same ribotype-RAPD group were further separated into unique entities.
为了进行流行病学研究，对133株两种生物型的溶血巴斯德菌（分别为90株生物型A和43株生物型T）以及几乎所有血清型进行了核糖体分型、随机扩增多态性DNA（RAPD）分析和脉冲场凝胶电泳（PFGE）分析。生物型A菌株共发现15种记录为核糖体分型HA至HO的模式，其中核糖体分型HA、HC和HD最为常见（66株[74%]）；生物型T菌株观察到20种核糖体分型，命名为HA'至HT'，与生物型A菌株观察到的明显不同。RAPD分析分别为生物群A和生物群T产生了总共44种（命名为Rp1至Rp44）和15种（命名为Rp1'至Rp15'）独特的RAPD模式。数据分析表明，无论动物学或地理来源如何，不同血清型的生物型A菌株都可以观察到特定的核糖体分型-RAPD模式组合，而生物型T菌株则不然。PFGE在菌株鉴别方面似乎更有效，因为来自不同动物学或地理来源、具有相同核糖体分型-RAPD组的选定菌株进一步被分为独特的个体。
