Inbar M, Ben-Bassat H
Int J Cancer. 1976 Sep 15;18(3):293-7. doi: 10.1002/ijc.2910180305.
Twenty-five human hematopoietic cell lines established from patients with malignant and non-malignant diseases have been studied for membrane fluidity. The degree of fluidity in the surface membranes was quantitatively monitored by fluorescence polarization analysis of the fluorescence probe, 1,6-diphenyl 1,3,5-hexatriene, when embedded in the lipid region of the surface membrane of intact cells. The results have shown that cells derived from malignant diseases have a more fluid lipid layer in their surface membrane than cells derived from non-malignant disorders. It is, therefore, suggested that a quantitative analysis of fluidity differences in the cell surface membrane lipid core can be of value to the studies on basic differences between normal and malignant cell lines established from the human hematopoietic system.
对从患有恶性和非恶性疾病的患者身上建立的25种人类造血细胞系进行了膜流动性研究。通过对荧光探针1,6 - 二苯基 - 1,3,5 - 己三烯嵌入完整细胞表面膜脂质区域时的荧光偏振分析，定量监测表面膜的流动性程度。结果表明，源自恶性疾病的细胞其表面膜中的脂质层比源自非恶性疾病的细胞更具流动性。因此，有人提出，对细胞表面膜脂质核心流动性差异进行定量分析，可能对研究从人类造血系统建立的正常和恶性细胞系之间的基本差异具有价值。
