Levanon A, Kohn A, Inbar M
J Virol. 1977 May;22(2):353-60. doi: 10.1128/JVI.22.2.353-360.1977.
Changes in the dynamic behavior of membrane lipids of mammalian cells induced by adsorption of animal viruses were quantitatively monitored by fluorescence polarization analysis with the aid of the fluorescent probe 1,6-diphenyl 1,3,5-hexatriene embedded in the surface membrane lipid core of intact cells. Adsorption of encephalomyocarditis, West Nile, and polyoma viruses to hamster (baby hamster kidney) and mouse (3T3) cells is accompanied by a rapid and significant increase in the degree of fluidity of membrane lipids of the infected cells. These changes in membrane fluidity, which are virus dose dependent, are inhibited by low temperature and by treatment of the cells before-hand with compounds known to block viral receptors on the cell surface. It is suggested that increase in membrane lipid fluidity, induced by the adsorption of virions, is an early event in the process of cell-virus interactions.
借助嵌入完整细胞表面膜脂核心的荧光探针1,6-二苯基-1,3,5-己三烯，通过荧光偏振分析对动物病毒吸附诱导的哺乳动物细胞膜脂动态行为变化进行了定量监测。脑心肌炎病毒、西尼罗河病毒和多瘤病毒吸附到仓鼠（幼仓鼠肾）和小鼠（3T3）细胞上，会伴随着被感染细胞膜脂流动性程度迅速且显著增加。这些膜流动性的变化与病毒剂量有关，低温以及预先用已知可阻断细胞表面病毒受体的化合物处理细胞可抑制这种变化。有人提出，病毒粒子吸附诱导的膜脂流动性增加是细胞 - 病毒相互作用过程中的早期事件。
