Ohya T, Kudo N, Suzuki E, Kawashima Y
Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
J Chromatogr B Biomed Sci Appl. 1998 Dec 11;720(1-2):1-7. doi: 10.1016/s0378-4347(98)00448-4.
This paper describes a method for the quantitative determination of perfluorinated carboxylic acids (PFCAs), perfluorohexanoic acid (C6-PFCA), perfluoroheptanoic acid (C7-PFCA), perfluorooctanoic acid (C8-PFCA), perfluorononanoic acid (C9-PFCA) and perfluorodecanoic acid (C10-PFCA), in biological samples. PFCA in liver homogenates was extracted as an ion pair with tetrabutylammonium (TBA) ion into organic solvent, then the PFCA was derivatized with 3-bromoacetyl-7-methoxycoumarin (BrAMC) and quantified by HPLC with fluorescence detection. This method is applicable for the studies on tissue accumulation and elimination of PFCAs in animals after the administration.
本文描述了一种用于定量测定生物样品中全氟羧酸（PFCA）、全氟己酸（C6-PFCA）、全氟庚酸（C7-PFCA）、全氟辛酸（C8-PFCA）、全氟壬酸（C9-PFCA）和全氟癸酸（C10-PFCA）的方法。肝脏匀浆中的PFCA与四丁基铵（TBA）离子形成离子对后被萃取到有机溶剂中，然后PFCA用3-溴乙酰基-7-甲氧基香豆素（BrAMC）进行衍生化，并通过带有荧光检测的高效液相色谱法进行定量。该方法适用于研究动物给药后PFCA在组织中的蓄积和消除情况。
