Poboży Ewa, Król Edyta, Wójcik Lena, Wachowicz Mariusz, Trojanowicz Marek
Mikrochim Acta. 2011 Mar;172(3-4):409-417. doi: 10.1007/s00604-010-0513-z. Epub 2010 Dec 12.
Perfluorinated carboxylic acids (PFCAs) represent an important group of persistent perfluorinated organic compounds commonly determined in environmental and biological samples. A reversed-phase HPLC method was developed based on derivatization of the PFCAs with the commercially available fluorescent reagent 3-bromoacetyl coumarin. The method was optimized and this resulted in the efficient separation of PFCAs containing from 3 to 12 carbon atoms in molecule in 25 min run. To improve sensitivity, the preconcentration step has been optimized using Oasis-WAX and C18 sorbents for SPE. A 100-fold preconcentration is achieved by solid-phase extraction with the sorbent C18 Sep-PAK to result in limits of detection in the range from 43 to 75 ppt for the analytes examined, and in the application of the method of water analysis.FigureChromatogram of mixture of perfluorinated carboxylic acids C3-PFCA - C12-PFCA with fluorescence detection after derivatization with 3-bromoacetyl coumarin (b), and blank (a).
全氟羧酸（PFCAs）是一类重要的持久性全氟有机化合物，常在环境和生物样品中检测到。基于使用市售荧光试剂3-溴乙酰香豆素对PFCAs进行衍生化，开发了一种反相高效液相色谱法。该方法经过优化，在25分钟的运行时间内实现了分子中含有3至12个碳原子的PFCAs的有效分离。为提高灵敏度，使用Oasis-WAX和C18吸附剂进行固相萃取（SPE）对预浓缩步骤进行了优化。通过使用C18 Sep-PAK吸附剂进行固相萃取实现了100倍的预浓缩，使得所检测分析物的检测限在43至75 ppt范围内，并应用于水分析方法中。图 用3-溴乙酰香豆素衍生化后具有荧光检测的全氟羧酸C3-PFCA - C12-PFCA混合物的色谱图（b）和空白（a）。
