Whitham S A, Yamamoto M L, Carrington J C
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):772-7. doi: 10.1073/pnas.96.2.772.
The genetic basis for susceptibility or nonsusceptibility of plants to viruses is understood poorly. Two selectable tobacco etch virus (TEV) strains were developed for identification of Arabidopsis thaliana mutants with either gain-of-susceptibility or loss-of-susceptibility phenotypes. These strains conferred a conditional-survival phenotype to Arabidopsis based on systemic expression of herbicide resistance or proherbicide sensitivity genes, thereby facilitating mass selections and screens for Arabidopsis mutants that enhance or suppress TEV replication, cell-to-cell movement, or long-distance movement. A multicomponent mechanism that restricts systemic invasion of TEV was identified through isolation of gain-of-susceptibility mutants with alterations at two loci.
植物对病毒易感性或非易感性的遗传基础目前了解甚少。我们开发了两种可选择的烟草蚀纹病毒（TEV）株系，用于鉴定具有易感性增强或易感性丧失表型的拟南芥突变体。这些株系基于除草剂抗性或前体除草剂敏感性基因的系统性表达，赋予拟南芥一种条件存活表型，从而便于大规模筛选和鉴定增强或抑制TEV复制、细胞间移动或长距离移动的拟南芥突变体。通过分离在两个位点发生改变的易感性增强突变体，确定了一种限制TEV系统性侵染的多组分机制。
