Tokuç G, Doğan O, Ayan I, Kebudi R, Doğan S, Demiryont M, Camlica H
Institute of Oncology, Department of Pediatric Oncology, University of Istanbul, Turkey.
Acta Paediatr Jpn. 1998 Dec;40(6):573-9. doi: 10.1111/j.1442-200x.1998.tb01993.x.
The levels of proliferating cell nuclear antigen (PCNA) are almost negligible in long-term quiescent cells and increase dramatically during the cell cycle. Recently, the monoclonal antibodies to PCNA have been used to demonstrate the proliferative component of paraffin-embedded tumor tissues. It has been shown to be available as a simple histological marker of proliferative activity and the PCNA labeling index has been correlated with the prognosis of several malignant neoplasms.
Formalin fixed, paraffin embedded tissue specimens of 29 primary pediatric rhabdomyosarcomas were immunostained by using an anti-PCNA monoclonal antibody (DAKO PCNA PC10). The relationship between the PCNA index and prognosis, clinicopathological features and survival were assessed retrospectively.
The mean PCNA index for the whole series was 54%. There was no correlation between PCNA index and any of the clinicopathological characteristics. However, patients having tumors with a high (> 54%) PCNA index demonstrated significantly lower survival rates than tumors with a low (< 54%) PCNA index (P = 0.01). Moreover, there were significantly more patients with relapse or progressive disease in the high PCNA index group (P = 0.005).
The PCNA labeling index can be a useful prognostic factor and a good indicator of recurrence and/or survival in patients with rhabdomyosarcoma.
增殖细胞核抗原（PCNA）在长期静止细胞中的水平几乎可以忽略不计，而在细胞周期中会急剧增加。最近，针对PCNA的单克隆抗体已被用于显示石蜡包埋肿瘤组织的增殖成分。它已被证明是一种简单的增殖活性组织学标志物，并且PCNA标记指数与几种恶性肿瘤的预后相关。
使用抗PCNA单克隆抗体（DAKO PCNA PC10）对29例原发性小儿横纹肌肉瘤的福尔马林固定、石蜡包埋组织标本进行免疫染色。回顾性评估PCNA指数与预后、临床病理特征及生存率之间的关系。
整个系列的平均PCNA指数为54%。PCNA指数与任何临床病理特征之间均无相关性。然而，PCNA指数高（>54%）的肿瘤患者的生存率明显低于PCNA指数低（<54%）的肿瘤患者（P = 0.01）。此外，PCNA指数高的组中复发或疾病进展的患者明显更多（P = 0.005）。
PCNA标记指数可以作为横纹肌肉瘤患者有用的预后因素以及复发和/或生存的良好指标。
