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A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermatophagoides pteronyssinus.一种用于测量来自尘螨嗜热栖尘螨的主要变应原Der p 1的半胱氨酸蛋白酶活性的灵敏荧光测定法。
Mol Pathol. 1998 Aug;51(4):222-4. doi: 10.1136/mp.51.4.222.
2
The isolation and characterization of a novel collagenolytic serine protease allergen (Der p 9) from the dust mite Dermatophagoides pteronyssinus.
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Der p 1 is the primary activator of Der p 3, Der p 6 and Der p 9 the proteolytic allergens produced by the house dust mite Dermatophagoides pteronyssinus.Der p 1是Der p 3、Der p 6和Der p 9的主要激活剂，这三种物质是屋尘螨嗜肺螨形螨产生的蛋白水解性过敏原。
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The cysteine protease activity of the major dust mite allergen Der p 1 selectively enhances the immunoglobulin E antibody response.主要尘螨过敏原Der p 1的半胱氨酸蛋白酶活性选择性增强免疫球蛋白E抗体反应。
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Isolation of cysteine protease in the crude mite extract, Dermatophagoides farinae.
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Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface-exposed residues that may influence antibody binding.螨虫过敏原Der f 1和Der p 1的晶体结构揭示了可能影响抗体结合的表面暴露残基的差异。
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9
Activation mechanism of recombinant Der p 3 allergen zymogen: contribution of cysteine protease Der p 1 and effect of propeptide glycosylation.重组变应原Der p 3酶原的激活机制：半胱氨酸蛋白酶Der p 1的作用及前肽糖基化的影响
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10
The cysteine protease activity of the major dust mite allergen Der p 1 selectively enhances the immunoglobulin E antibody response.主要尘螨过敏原Der p 1的半胱氨酸蛋白酶活性选择性增强免疫球蛋白E抗体反应。
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本文引用的文献

1
On the potential significance of the enzymatic activity of mite allergens to immunogenicity. Clues to structure and function revealed by molecular characterization.关于螨过敏原酶活性对免疫原性的潜在意义。分子特征揭示的结构与功能线索。
Clin Exp Allergy. 1997 Jan;27(1):10-21.
2
Cleavage of the low-affinity receptor for human IgE (CD23) by a mite cysteine protease: nature of the cleaved fragment in relation to the structure and function of CD23.螨半胱氨酸蛋白酶对人IgE低亲和力受体（CD23）的裂解作用：裂解片段的性质与CD23结构和功能的关系
Eur J Immunol. 1997 Mar;27(3):584-8. doi: 10.1002/eji.1830270303.
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Is the dust mite allergen Der p1 a cysteine proteinase?
Biochem Soc Trans. 1997 Feb;25(1):85S. doi: 10.1042/bst025085s.
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The ataxia-telangiectasia gene product, a constitutively expressed nuclear protein that is not up-regulated following genome damage.共济失调毛细血管扩张症基因产物，一种组成性表达的核蛋白，在基因组损伤后不会上调。
Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1840-5. doi: 10.1073/pnas.94.5.1840.
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Differential subcellular localization, expression and biological toxicity of BRCA1 and the splice variant BRCA1-delta11b.BRCA1 及其剪接变体 BRCA1-δ11b 的亚细胞定位差异、表达及生物毒性
Oncogene. 1997 Jan 9;14(1):1-16. doi: 10.1038/sj.onc.1200924.
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Potential allergens stimulate the release of mediators of the allergic response from cells of mast cell lineage in the absence of sensitization with antigen-specific IgE.在没有抗原特异性IgE致敏的情况下，潜在变应原可刺激肥大细胞系细胞释放过敏反应介质。
Eur J Immunol. 1996 Dec;26(12):2972-80. doi: 10.1002/eji.1830261224.
7
Molecular genotyping shows that ataxia-telangiectasia heterozygotes are predisposed to breast cancer.分子基因分型显示，共济失调毛细血管扩张症杂合子易患乳腺癌。
Cancer Genet Cytogenet. 1996 Dec;92(2):130-4. doi: 10.1016/s0165-4608(96)00328-7.
8
The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.Atr和Atm蛋白激酶与减数分裂配对染色体上的不同位点相关联。
Genes Dev. 1996 Oct 1;10(19):2423-37. doi: 10.1101/gad.10.19.2423.
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Cellular responses to DNA damage: cell-cycle checkpoints, apoptosis and the roles of p53 and ATM.细胞对DNA损伤的反应：细胞周期检查点、细胞凋亡以及p53和ATM的作用。
Trends Biochem Sci. 1995 Oct;20(10):426-30. doi: 10.1016/s0968-0004(00)89093-3.
10
The distribution of amyloid plaques in the cerebellum and brain stem in Down's syndrome and Alzheimer's disease: a light microscopical analysis.唐氏综合征和阿尔茨海默病中小脑和脑干中淀粉样斑块的分布：光镜分析
Acta Neuropathol. 1993;85(5):542-52. doi: 10.1007/BF00230495.

一种用于测量来自尘螨嗜热栖尘螨的主要变应原Der p 1的半胱氨酸蛋白酶活性的灵敏荧光测定法。

A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermatophagoides pteronyssinus.

作者信息

Schulz O, Sewell H F, Shakib F

机构信息

Division of Molecular and Clinical Immunology, Faculty of Medicine and Health Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, UK.

出版信息

Mol Pathol. 1998 Aug;51(4):222-4. doi: 10.1136/mp.51.4.222.

DOI:10.1136/mp.51.4.222
PMID:9893750
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC395641/
Abstract

The potent allergenicity of Der p 1, a major allergen of the house dust mite Dermatophagoides pteronyssinus, is thought to be related to its cysteine protease activity. Therefore, there is considerable interest in developing a sensitive assay for measuring Der p 1 activity to screen for specific inhibitors. This study demonstrates for the first time that the activity of Der p 1 can be measured conveniently in a continuous rate assay with the fluorogenic substrate Boc-Gln-Ala-Arg-AMC (K(m) = 280 microM and kcat/K(m) = 4.6 x 10(3)/M/s).

摘要

屋尘螨嗜热栖尘螨的主要变应原Der p 1具有很强的变应原性，其被认为与半胱氨酸蛋白酶活性有关。因此，人们对开发一种灵敏的测定方法来检测Der p 1活性以筛选特异性抑制剂有着浓厚兴趣。本研究首次证明，使用荧光底物Boc-Gln-Ala-Arg-AMC（米氏常数K(m)=280微摩尔，催化常数与米氏常数的比值kcat/K(m)=4.6×10³/摩尔/秒），可以通过连续速率测定法方便地检测Der p 1的活性。