Schimpff R M, Donnadieu M, Glasinovic J C, Warnet J M, Girard F
Acta Endocrinol (Copenh). 1976 Oct;83(2):365-72. doi: 10.1530/acta.0.0830365.
Somatomedin (Sm) activity (measured by [35S] utake in chick embryo cartilage) was determined in serum samples simultaneously drawn from the hepatic vein, portal vein, femoral artery and demoral vein of seventeen anaesthezied normal adult dogs). A pool of human serum was taken as reference (Sm = 1 U/ml). Sm levels in the peripheral vein of dogs were 0.38 +/- 0.03 U/ml). Mean +/- SEM). Sm activity was greater in the hepatic vein (0.48 U/ml) than in the other vessels (0.36, 0.39, 0.38 U/ml), and the paired differences were significant (P less than 0.002 to P less than 0.05). In three dogs which received b-GH (20 IU/day), the Sm levels were significantly increased after nine days in the femoural vein (P less than 0.05) and in the hepatic vein (P less than 0.05). The validity of the assay is discussed; a possible interference of NEFA in the assay is eliminated. The difference of Sm levels between hepatic and portal veins, related to hepatic flow measured in seven of these dogs, indicate an important production of Sm by the liver.
测定了17只麻醉状态下的正常成年犬从肝静脉、门静脉、股动脉和股静脉同时采集的血清样本中的生长调节素（Sm）活性（通过鸡胚软骨对[35S]的摄取来测量）。取一份人血清作为对照（Sm = 1 U/ml）。犬外周静脉中的Sm水平为0.38±0.03 U/ml（均值±标准误）。肝静脉中的Sm活性（0.48 U/ml）高于其他血管（0.36、0.39、0.38 U/ml），配对差异具有显著性（P<0.002至P<0.05）。在3只接受b - GH（20 IU/天）的犬中，9天后股静脉（P<0.05）和肝静脉（P<0.05）中的Sm水平显著升高。讨论了该检测方法的有效性；消除了游离脂肪酸（NEFA）在检测中可能的干扰。在其中7只犬中，肝静脉和门静脉之间Sm水平的差异与所测肝血流量相关，这表明肝脏大量产生Sm。
