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磷-31核磁共振检测肌肉中意外的磷酸二酯。

Phosphorus-31 nuclear magnetic resonance detection of unexpected phosphodiesters in muscle.

作者信息

Burt C T, Glonek T, Bárány M

出版信息

Biochemistry. 1976 Nov 2;15(22):4850-3. doi: 10.1021/bi00667a015.

DOI:10.1021/bi00667a015
PMID:990247
Abstract

In the examination of intact muscles by 31P nuclear magnetic resonance spectroscopy, a number of signals have been detected in the phosphodiester region (-0.5 to 0.5 ppm) of the spectrum which could not be correlated with the known common phosphates of muscle tissue. These signals arise from perchloric acid extractable compounds with several common chemical properties, one of which is a ready solubility in nearly anhydrous ethanol solutions. A component contributing to the major resonance has been identified as glycerol-3-phosphorylcholine. This characterization is based on both 31P nuclear magnetic resonance and chromatographic data.

摘要

在用31P核磁共振波谱法检测完整肌肉时,在光谱的磷酸二酯区域(-0.5至0.5 ppm)检测到了一些信号,这些信号与肌肉组织中已知的常见磷酸盐不相关。这些信号来自具有几种常见化学性质的高氯酸可提取物,其中之一是易溶于几乎无水的乙醇溶液。对主要共振有贡献的一种成分已被鉴定为甘油-3-磷酸胆碱。这一表征基于31P核磁共振和色谱数据。

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