水合作用对人体红细胞含水量的影响。
Effect of hydration on the water content of human erythrocytes.
作者信息
Levin R L, Cravalho E G, Huggins C E
出版信息
Biophys J. 1976 Dec;16(12):1411-26. doi: 10.1016/S0006-3495(76)85784-0.
Abstract
An ideal, hydrated, nondilute pseudobinary salt-protein-water solution model of the RBC intracellular solution has been developed to describe the osmotic behavior of human erythrocytes during freezing and thawing. Because of the hydration of intracellular solutes (mostly cell proteins), our analytical results predict that at least 16.65% of the isotonic cell water content will be retained within RBCs placed in hypertonic solutions. These findings are consistent not only with the experimental measurements of the amount of isotonic cell water retained within RBCs subjected to nonisotonic extracellular solutions (20-32%) but also with the experimental evidence that all of the water within RBCs is solvent water. By modeling the RBC intracellular solution as a hydrated salt-protein-water solution, no anomalous osmotic behavior is apparent.
摘要
已经建立了一种理想的、水合的、非稀释的假二元盐 - 蛋白质 - 水溶液模型来描述人红细胞在冷冻和解冻过程中的渗透行为。由于细胞内溶质（主要是细胞蛋白质）的水合作用，我们的分析结果预测，置于高渗溶液中的红细胞将保留至少16.65%的等渗细胞含水量。这些发现不仅与在非等渗细胞外溶液中红细胞内保留的等渗细胞水量的实验测量结果（20 - 32%）一致，而且与红细胞内所有水都是溶剂水的实验证据一致。通过将红细胞内溶液建模为水合盐 - 蛋白质 - 水溶液，未观察到异常的渗透行为。
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