Huner N P, Macdowall D H
Can J Biochem. 1976 Oct;54(10):848-53. doi: 10.1139/o76-122.
Soluble proteins and membrane polypeptides were separated from chloroplasts isolated intact from a cultivar each of spring wheat, winter wheat, and more freeze-resistant rye, and changes in them associated with cold hardening were detected by means of polyacrylamide gel electrophoresis. No drastic changes in chloroplast membrane polypeptides occurred during growth at low temperatures in the three cultivars. However, subtle changes were evident in the soluble chloroplast protein fraction. In this fraction at least one varietal difference was discernable, yet all cultivars produced a new protein band of lowest mobility during growth at low temperatures. After the preparations were fractionated by Sephadex G-50 all unhardened plant material displayed two peaks in the region of the fraction I protein band, whereas all cold-hardening material displayed one peak. A different band of soluble protein was present only after cold hardening in Kharkov whear and Puma rye, and was not present in extracts from the cold-grown spring wheat.
从春小麦、冬小麦各一个品种以及抗冻性更强的黑麦中完整分离出叶绿体，从中分离出可溶性蛋白质和膜多肽，并通过聚丙烯酰胺凝胶电泳检测它们与抗寒锻炼相关的变化。在三个品种中，低温生长期间叶绿体膜多肽没有发生剧烈变化。然而，可溶性叶绿体蛋白部分有明显的细微变化。在这一部分中至少可以看出一个品种差异，但所有品种在低温生长期间都产生了一条迁移率最低的新蛋白带。用葡聚糖凝胶G - 50对样品进行分级分离后，所有未进行抗寒锻炼的植物材料在Ⅰ类蛋白带区域显示出两个峰，而所有抗寒锻炼材料显示出一个峰。只有哈尔科夫小麦和彪马黑麦经过抗寒锻炼后才出现一条不同的可溶性蛋白带，而冷生长的春小麦提取物中没有这条带。
