Kraus A, Dessau W, Simon H
Hoppe Seylers Z Physiol Chem. 1976 Sep;357(9):1209-14. doi: 10.1515/bchm2.1976.357.2.1209.
During the transformation of (2S, 3R) [3-3H]malate to (S) lactate no tritium exchange takes place. The stereochemical course of the decarboxylation studied with (2S, 3R) [3-2H]-malate in 3HOH/H2O and (2S, 3R) [3-3H]malate in 2H2O occurs with retention and is therefore the same as that determined by other authors for malic enzyme from vertebrates and from Escherichia coli. The malate-lactate fermentation is a useful procedure to prepare chiral methyl groups on a preparative scale starting from (2S, 3R) [3-H]malate.
在(2S, 3R) [3-³H]苹果酸向(S)乳酸的转化过程中，没有发生氚交换。用(2S, 3R) [3-²H]苹果酸在³H₂O/H₂O体系以及(2S, 3R) [3-³H]苹果酸在²H₂O中研究的脱羧反应的立体化学过程是构型保持的，因此与其他作者对脊椎动物和大肠杆菌的苹果酸酶所确定的过程相同。苹果酸-乳酸发酵是一种从(2S, 3R) [3-H]苹果酸出发在制备规模上制备手性甲基的有用方法。
