广藿香轻度花叶病毒（一种新型黄病毒属病毒）RNA2的基因组组织的完整核苷酸序列

Complete nucleotide sequence of the genome organization of RNA2 of patchouli mild mosaic virus, a new favavirus.

作者信息

Ikegami M, Kawashima H, Natsuaki T, Sugimura N

机构信息

Department of Bioscience, Tokyo University of Agriculture, Japan.

出版信息

Arch Virol. 1998;143(12):2431-4. doi: 10.1007/s007050050472.

DOI:10.1007/s007050050472

PMID:9930198

Abstract

The complete nucleotide sequence and the genome organization of the RNA 2 of a patchouli mild mosaic virus (PaMMV) was determined. The sequence consists of 3591 nucleotides and contains a single long open reading frame sufficient to code for 118 K protein. Three proteins of 52 K, 44 K and 22 K could be encoded by the PaMMV RNA 2 genome. Our analysis of the N-terminal sequences of two species of coat protein (CP) allowed precise location of the CP cistrons within the polyprotein. 44 K and 22 K proteins are the coat proteins. The positions of the cleavage sites are Gln/Ala between 44 K and 22 K coat proteins and Gln/Gly between 52 K and 44 K proteins. Comparison of PaMMV RNA 2 with comoviral and nepoviral RNA 2 showed no sequence similarity. These results as well as previous serological studies strongly suggest that PaMMV is a member in the genus Fabavirus.

摘要

我们测定了广藿香轻度花叶病毒（PaMMV）RNA 2的完整核苷酸序列和基因组结构。该序列由3591个核苷酸组成，包含一个单一的长开放阅读框，足以编码118K蛋白。PaMMV RNA 2基因组可编码三种蛋白质，分别为52K、44K和22K。我们对两种外壳蛋白（CP）的N端序列进行分析，从而能够精确确定CP顺反子在多蛋白中的位置。44K和22K蛋白为外壳蛋白。切割位点分别位于44K和22K外壳蛋白之间的Gln/Ala处，以及52K和44K蛋白之间的Gln/Gly处。将PaMMV RNA 2与雀麦花叶病毒属病毒和线虫传多面体病毒属病毒的RNA 2进行比较，未发现序列相似性。这些结果以及先前的血清学研究强烈表明，PaMMV是蚕豆病毒属的一个成员。