Parodi P W
J Dairy Sci. 1976 Nov;59(11):1870-3. doi: 10.3168/jds.s0022-0302(76)84455-4.
Milk fat octadecenoic acids were isolated by preparative gas-liquid chromatography of their methyl esters and separated into cis and trans isomers by argentation thin-layer chromatography. The distribution of the double bonds in the two fractions was determined by reductive ozonolysis. Cis octadecenoic acids had double bonds in positions 6 to 14. The cis-9 isomer was the most abundant isomer representing approximately 95% of the total. Trans octadecenoic acids had double bonds in positions 6 to 16, the predominant isomer being trans-11 octadecenoic acid. Data illustrating the range of variation in the distribution of positional isomers of octadecenoic acid for herd milk fat and butter are presented. There was no obvious seasonal variation in the distribution pattern. Fat from perirenal and subcutaneous adipose tissue of a dairy cow had composition similar to that of butter samples.
乳脂肪十八碳烯酸通过其甲酯的制备气相色谱法分离，并通过银化薄层色谱法分离为顺式和反式异构体。通过还原臭氧化法测定两个馏分中双键的分布。顺式十八碳烯酸在6至14位具有双键。顺式-9异构体是最丰富的异构体，约占总量的95%。反式十八碳烯酸在6至16位具有双键，主要异构体是反式-11十八碳烯酸。给出了说明牛群乳脂肪和黄油中十八碳烯酸位置异构体分布变化范围的数据。分布模式没有明显的季节性变化。奶牛肾周和皮下脂肪组织的脂肪组成与黄油样品相似。
