Maier K P, Talke H, Hoppe-Seyler G, Fröhlich J, Schollmeyer P, Schönbach G, Erhart K P, Gerok W
Klin Wochenschr. 1976 Nov 1;54(21):1021-5. doi: 10.1007/BF01469247.
Enzymes of the Krebs-Henseleit urea-cycle were localized by means of differential centrifugation and fractional tissue extraction in rat liver and in human liver. Argininosuccinatlyase (ASAL) and Argininosuccinatsynthetase (ASAS) represent enzymes of the soluble cytoplasmic fraction. Ornithine-ketoacid-transaminase(OKT), carbamyl-phosphate-synthetase (CPS) and ornithine-carbamyl-transferase (OCT) are localized in the mitochondrial and nuclei fractions of the liver cell. Most of the arginase activity is bound to subcellular structures (probably to nuclei). A small portion of arginase-activity was found in the soluble cytoplasmatic fraction. The enzymes of the Krebs-Henseleit urea-cycle are equally distributed in rat liver and in human liver. Differences in the subcellular localisation of (mitochondrial) enzymes in human liver could be attributed to mitochondrial breakage during tissue preparation and do not represent in-vivo conditions.
通过差速离心和组织分级提取法，对大鼠肝脏和人肝脏中克雷布斯 - 亨斯莱特尿素循环的酶进行了定位研究。精氨琥珀酸裂解酶（ASAL）和精氨琥珀酸合成酶（ASAS）属于可溶性细胞质部分的酶。鸟氨酸 - 酮酸转氨酶（OKT）、氨基甲酰磷酸合成酶（CPS）和鸟氨酸氨基甲酰转移酶（OCT）定位于肝细胞的线粒体和细胞核部分。大部分精氨酸酶活性与亚细胞结构（可能是细胞核）结合。在可溶性细胞质部分发现了一小部分精氨酸酶活性。克雷布斯 - 亨斯莱特尿素循环的酶在大鼠肝脏和人肝脏中的分布是相同的。人肝脏中（线粒体）酶的亚细胞定位差异可能归因于组织制备过程中的线粒体破裂，并不代表体内情况。
