Nazzaro-Porro M, Passi S
J Invest Dermatol. 1978 Sep;71(3):205-8. doi: 10.1111/1523-1747.ep12547184.
Lipid fractions capable of inhibiting the dopa-tyrosinase reaction in vitro were isolated by thin-layer chromatography from submerged cultures of Pityrosporum supplemented with oleic acid or vaccenic acid. Analysis of these fractions by gas chromatography-mass spectrometry revealed the presence mainly of C(9) and C(11) dicarboxylic acids. Standard dicarboxylic acids from C(8) to C(13) were capable of inhibiting tyrosinase in vitro to varying extents. Enzymatic kinetic studies showed that they act as competitive inhibitors of tyrosinase. These observations suggest that dicarboxylic acids could be used in the treatment of people with hyperpigmentary disorders.
通过薄层色谱法从添加油酸或牛痘酸的马拉色菌深层培养物中分离出能够在体外抑制多巴 - 酪氨酸酶反应的脂质组分。气相色谱 - 质谱联用仪对这些组分的分析表明，主要存在C(9)和C(11)二羧酸。从C(8)到C(13)的标准二羧酸能够在体外不同程度地抑制酪氨酸酶。酶动力学研究表明，它们作为酪氨酸酶的竞争性抑制剂起作用。这些观察结果表明，二羧酸可用于治疗色素沉着过度症患者。
