Li H, Andersson E M, Helander H F
Preclinical R&D, Astra Hässle AB, Mölndal, Sweden.
Dig Dis Sci. 1999 Jan;44(1):116-24. doi: 10.1023/a:1026662402734.
The aim of the present study was to investigate responses from the gastric mucosa of rats during long-term H. pylori infection. Twenty-four Sprague-Dawley rats were inoculated with a mouse-adapted strain of human H. pylori (vacA+, cagA+), 16 uninfected rats served as controls. Three to six rats from each group were killed two weeks or two, six, or 12 months later. At sacrifice, blood was sampled and the gastric mucosa was taken for bacterial culture, histology, immunocytochemistry and in situ hybridization. H. pylori colonized the antrum in 23/24 inoculated rats; with time the density of bacteria increased. The inflammation in the antral mucosa was mild to moderate and was dominated by infiltration of lymphocytes and macrophages. Serum H. pylori-specific IgG2a was significantly increased in the infected rats. The frequency of epithelial cell apoptosis was significantly increased in the early months of infection. The mucosal expression of trefoil peptide mRNA remained unchanged. We conclude that after one year of H. pylori infection in rats, the mucosal responses were rather mild, indicating that the animals may adapt to the infection by mechanisms which remain to be identified.
本研究的目的是调查大鼠长期幽门螺杆菌感染期间胃黏膜的反应。24只Sprague-Dawley大鼠接种了人源幽门螺杆菌的小鼠适应株（vacA+，cagA+），16只未感染大鼠作为对照。每组3至6只大鼠在2周或2、6或12个月后处死。处死时，采集血液并取胃黏膜进行细菌培养、组织学、免疫细胞化学和原位杂交。23/24只接种大鼠的胃窦部出现幽门螺杆菌定植；随着时间推移，细菌密度增加。胃窦黏膜炎症为轻度至中度，以淋巴细胞和巨噬细胞浸润为主。感染大鼠血清幽门螺杆菌特异性IgG2a显著升高。感染初期上皮细胞凋亡频率显著增加。三叶肽mRNA的黏膜表达保持不变。我们得出结论，大鼠幽门螺杆菌感染一年后，黏膜反应相当轻微，表明动物可能通过有待确定的机制适应感染。
